Vitellogenin from the Silkworm, Bombyx mori: An Effective Anti-Bacterial Agent
Open Access
- 13 September 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (9), e73005
- https://doi.org/10.1371/journal.pone.0073005
Abstract
Silkworm, Bombyx mori, vitellogenin (Vg) was isolated from perivisceral fat body of day 3 of pupa. Both Vg subunits were co-purified as verified by mass spectrometry and immunoblot. Purified Vg responded to specific tests for major posttranslational modifications on native gels indicating its nature as lipo-glyco-phosphoprotein. The Vg fraction had strong antibacterial activity against Gram negative bacterium Escherichia coli and Gram positive bacterium Bacillus subtilis. Microscopic images showed binding of Vg to bacterial cells and their destruction. When infected silkworm larvae were treated with purified Vg they survived the full life cycle in contrast to untreated animals. This result showed that Vg has the ability to inhibit the proliferation of bacteria in the silkworm fluid system without disturbing the regular metabolism of the host.This publication has 42 references indexed in Scilit:
- Vitellogenin, a multivalent sensor and an antimicrobial effectorThe International Journal of Biochemistry & Cell Biology, 2011
- Deconstructing honeybee vitellogenin: novel 40 kDa fragment assigned to its N terminusJournal of Experimental Biology, 2011
- Molecular diversity and evolution of the large lipid transfer protein superfamilyJournal of Lipid Research, 2007
- Receptor-Ligand Interaction between Vitellogenin Receptor (VtgR) and Vitellogenin (Vtg), Implications on Low Density Lipoprotein Receptor and Apolipoprotein B/EJournal of Biological Chemistry, 2003
- Receptor mediated yolk protein uptake in the crab Scylla serrata: crustacean vitellogenin receptor recognizes related mammalian serum lipoproteinsMolecular Reproduction and Development, 2002
- Vitellogenin gene of the silkworm, Bombyx mori: Structure and sex‐dependent expressionFEBS Letters, 1994
- Structure and expression of mRNA for vitellogenin in Bombyx moriBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1994
- Vitellogenin and Vitellin in InsectsAnnual Review of Entomology, 1979
- The synthesis of vitellogenin in the Cecropia silkwormJournal of Insect Physiology, 1971
- Vitellogenic Blood Protein Synthesis by Insect Fat BodyScience, 1969