Transcriptional Activation Modulated by Homopolymeric Glutamine and Proline Stretches
- 11 February 1994
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 263 (5148), 808-811
- https://doi.org/10.1126/science.8303297
Abstract
Many transcription factors contain proline- or glutamine-rich activation domains. Here it is shown that simple homopolymeric stretches of these amino acids can activate transcription when fused to the DNA binding domain of GAL4 factor. In vitro, activity increased with polymer length, whereas in cell transfection assays maximal activity was achieved by 10 to 30 glutamines or about 10 prolines. Similar results were obtained when glutamine stretches were placed within a [GAL4]-VP16 chimeric protein. Because these stretches are encoded by rapidly evolving triplet repeats (microsatellites), they may be the main cause for modulation of transcription factor activity and thus result in subtle or overt genomic effects.Keywords
This publication has 23 references indexed in Scilit:
- Mitotic stability and meiotic variability of the (CAG)n repeat in the Huntington disease geneHuman Molecular Genetics, 1993
- Trinucleotide repeat elongation in the Huntingtin gene in Huntington Disease patients from 71 Danish familiesHuman Molecular Genetics, 1993
- Genes with triplet repeats: candidate mediators of neuropsychiatric disordersTrends in Neurosciences, 1993
- Association of fragile X syndrome with delayed replication of the FMR1 geneCell, 1993
- A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomesCell, 1993
- Survey of human and rat microsatellitesGenomics, 1992
- Absence of expression of the FMR-1 gene in fragile X syndromeCell, 1991
- Physical mapping across the fragile X: Hypermethylation and clinical expression of the fragile X syndromeCell, 1991
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- opa: A novel family of transcribed repeats shared by the Notch locus and other developmentally regulated loci in D. melanogasterCell, 1985