Porcine reproductive and respiratory syndrome (PRRS) virus down-modulates TNF-α production in infected macrophages
- 10 August 2000
- journal article
- Published by Elsevier BV in Virus Research
- Vol. 69 (1), 41-46
- https://doi.org/10.1016/s0168-1702(00)00172-6
Abstract
The effect of porcine reproductive and respiratory syndrome (PRRS) virus infection on the synthesis and secretion of TNF-α and other pro-inflammatory cytokines by porcine alveolar macrophages (PAM) was investigated as well as the effect that TNF-α has on the replication of this virus. A clear reduction of phorbol myristate acetate (PMA)-induced expression of TNF–α mRNA was observed in cells incubated with PRRS virus. Moreover, the presence of PRRS virus also induced a decrease in IL-1α and MIP-1β mRNAs expression with respect to PMA-stimulated uninfected cells. According to these results, exposure to the PRRS virus led to a reduction of the TNF-α protein in supernatants of PMA-stimulated PAM. On the other hand, addition of recombinant porcine TNF-α to cultures clearly reduced virus replication; however the addition of TNF-α to cultures containing IFN-α did not result in a further reduction of the produced by IFN-α alone. This indicates the lack of synergy in the effect of these cytokines on viral replication.Keywords
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