Detection of Transient Interchain Interactions in the Intrinsically Disordered Protein α-Synuclein by NMR Paramagnetic Relaxation Enhancement
- 1 April 2010
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 132 (16), 5546-5547
- https://doi.org/10.1021/ja9105495
Abstract
NMR paramagnetic relaxation enhancement experiments were applied to the intrinsically disordered protein α-synuclein, the primary protein in Parkinson’s disease, to directly characterize transient intermolecular complexes at neutral and low pH. At neutral pH, we observed weak N- to C-terminal interchain contacts driven by electrostatic interactions, while at low pH, the C- to C-terminal interchain interactions are significantly stronger and driven by hydrophobic contacts. Characterization of these first interchain interactions will provide fundamental insight into the mechanism of amyloid formation.This publication has 18 references indexed in Scilit:
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