A microarray screen for novel candidate genes in coeliac disease pathogenesis
Open Access
- 1 July 2004
- Vol. 53 (7), 944-951
- https://doi.org/10.1136/gut.2003.018374
Abstract
Background and aims: The causative molecular pathways underlying the pathogenesis of coeliac disease are poorly understood. To unravel novel aspects of disease pathogenesis, we used microarrays to determine changes in gene expression of duodenal biopsies. Methods: cDNA microarrays representing 19 200 genes were used to compare gene expression profiles of duodenal biopsies from 15 coeliac disease patients with villous atrophy (Marsh III) and seven control individuals with normal biopsies (Marsh 0). In addition, the specific effect of gluten was studied by comparing the expression profiles of Marsh III lesions of seven patients exposed to gluten with four patients on a gluten free diet. Results: Comparing Marsh III with Marsh 0 lesions identified 109 genes that differed significantly (pConclusions: Our study provides new candidate genes in the pathogenesis of coeliac disease. Based on our results, we hypothesise that villous atrophy in coeliac disease patients is due to failure in cell differentiation. These genes are involved in pathways not previously implicated in coeliac disease pathogenesis and they may provide new targets for therapy.Keywords
This publication has 26 references indexed in Scilit:
- β-Catenin and TCF Mediate Cell Positioning in the Intestinal Epithelium by Controlling the Expression of EphB/EphrinBCell, 2002
- Upregulation of matrix metalloproteinases in a model of T cell mediated tissue injury in the gut: analysis by gene array and in situ hybridisationGut, 2002
- Structural Basis for Gluten Intolerance in Celiac SprueScience, 2002
- Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporinsThe EMBO Journal, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Molecular Basis of Celiac DiseaseAnnual Review of Immunology, 2000
- When is a coeliac a coeliac?Gut, 1998
- Scavenger Receptors Mediate Adhesion of Activated B LymphocytesExperimental Cell Research, 1998
- Gluten specific, HLA-DQ restricted T cells from coeliac mucosa produce cytokines with Th1 or Th0 profile dominated by interferon gamma.Gut, 1995
- A Tetraspan Membrane Glycoprotein Produced in the Human Intestinal Epithelium and Liver That Can Regulate Cell Density-dependent ProliferationPublished by Elsevier BV ,1995