Expression of spleen cell immunoglobulin phenotype in hybrids with myeloma cell lines
- 1 November 1981
- journal article
- Published by Springer Science and Business Media LLC in Somatic Cell and Molecular Genetics
- Vol. 7 (6), 657-666
- https://doi.org/10.1007/bf01538755
Abstract
Fusions were performed between myeloma cell lines, of mouse and rat origin, and mouse or rat spleen cells. Two statistical methods have been used to measure the proportion of hybrids expressing a spleen cellderived immunoglobulin phenotype, one of them applicable to cells growing under nonlimiting dilution conditions. The results indicate that there is strong preferential selection for hybrid cell growth with an immunoglobulin-secreting phenotype. The degree of preferential selection is dependent upon the myeloma cell line used and is most marked in the case of the rat myeloma lines. Surviving hybrids seem to originate from fusions of myeloma and spleen B (but not T) cells, but immunoglobulin production is lost more readily in certain combinations.Keywords
This publication has 16 references indexed in Scilit:
- Rat × rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgGNature, 1979
- Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reactionEuropean Journal of Immunology, 1977
- T lymphocyte tissue culture lines produced by cell hybridizationEuropean Journal of Immunology, 1977
- Hybrid cell lines with T-cell characteristicsNature, 1977
- Antibodies to major histocompatibility antigens produced by hybrid cell linesNature, 1977
- Derivation of specific antibody‐producing tissue culture and tumor lines by cell fusionEuropean Journal of Immunology, 1976
- Continuous cultures of fused cells secreting antibody of predefined specificityNature, 1975
- Fusion of Two Immunoglobulin-producing Myeloma CellsNature, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Selection of Hybrids from Matings of Fibroblasts in vitro and Their Presumed RecombinantsScience, 1964