Ultrasensitive Electrical Biosensing of Proteins and DNA: Carbon-Nanotube Derived Amplification of the Recognition and Transduction Events
Top Cited Papers
- 18 February 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (10), 3010-3011
- https://doi.org/10.1021/ja031723w
Abstract
A new strategy for dramatically amplifying enzyme-linked electrical detection of proteins and DNA using carbon nanotubes (CNTs) for carrying numerous enzyme tracers and accumulating the enzymatically liberated product on CNT-modified transducer is described. Such a CNT-derived double-step amplification pathway (of both the recognition and transduction events) allows the detection of DNA and proteins down to 1.3 and 160 zmol, respectively, in 25−50 μL samples and indicates great promise for PCR-free DNA analysis. The new protocol is illustrated for monitoring sandwich hybridization and antibody−antigen interactions in connection with alkaline-phosphatase tracers. The DNA-linking of CNTs and particles holds promise also for assembling hybrid nanostructures relevant to molecular electronic devices.Keywords
This publication has 13 references indexed in Scilit:
- Electrochemical DNA sensorsNature Biotechnology, 2003
- Enzyme-Amplified Amperometric Detection of 3000 Copies of DNA in a 10-μL Droplet at 0.5 fM ConcentrationAnalytical Chemistry, 2003
- Electrochemical Sensors Based on Carbon NanotubesElectroanalysis, 2002
- Electrochemical DNA Hybridization BiosensorsElectroanalysis, 2002
- Carbon Nanotubes--the Route Toward ApplicationsScience, 2002
- Electrochemical Transduction of Liposome-Amplified DNA SensingAngewandte Chemie-International Edition, 2000
- Towards Genoelectronics: Electrochemical Biosensing of DNA HybridizationChemistry – A European Journal, 1999
- Enzyme-Amplified Amperometric Detection of Hybridization and of a Single Base Pair Mutation in an 18-Base Oligonucleotide on a 7-μm-Diameter MicroelectrodeJournal of the American Chemical Society, 1999
- Ferrocenylethyl Phosphate: An Improved Substrate for the Detection of Alkaline Phosphatase by Cathodic Stripping Ion-Exchange Voltammetry. Application to the Electrochemical Enzyme Affinity Assay of AvidinAnalytical Chemistry, 1996
- Zeptomole-Detecting Biosensor for Alkaline Phosphatase in an Electrochemical Immunoassay for 2,4-Dichlorophenoxyacetic acidAnalytical Chemistry, 1996