Characterization of a Novel Lipoxygenase-Independent Senescence Mechanism in Alstroemeria peruviana Floral Tissue
Open Access
- 1 September 2002
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 130 (1), 273-283
- https://doi.org/10.1104/pp.000919
Abstract
The role of lipoxygenase (lox) in senescence ofAlstroemeria peruviana flowers was investigated using a combination of in vitro assays and chemical profiling of the lipid oxidation products generated. Phospholipids and galactolipids were extensively degraded during senescence in both sepals and petals and the ratio of saturated/unsaturated fatty acids increased. Lox protein levels and enzymatic activity declined markedly after flower opening. Stereochemical analysis of lox products showed that 13-lox was the major activity present in both floral tissues and high levels of 13-keto fatty acids were also synthesized. Lipid hydroperoxides accumulated in sepals, but not in petals, and sepals also had a higher chlorophyll to carotenoid ratio that favors photooxidation of lipids. Loss of membrane semipermeability was coincident for both tissue types and was chronologically separated from lox activity that had declined by over 80% at the onset of electrolyte leakage. Thus, loss of membrane function was not related to lox activity or accumulation of lipid hydroperoxides per se and differs in these respects from other ethylene-insensitive floral tissues representing a novel pattern of flower senescence.Keywords
This publication has 52 references indexed in Scilit:
- THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess PhotonsAnnual Review of Plant Physiology and Plant Molecular Biology, 1999
- Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compoundsPlanta, 1999
- Oxidative events during programmed cell death of daylily (Hemerocallis hybrid) petalsPlant Science, 1998
- Changes in respiration, protein and carbohydrates of tulip tepals and Alstroemeria petals during developmentJournal of Plant Physiology, 1997
- A shift of paradigm is needed in plant sciencePhysiologia Plantarum, 1997
- Ethylene and flower petal senescence: Interrelationship with membrane lipid catabolismPhysiologia Plantarum, 1997
- Antioxidant activities of carotenes and xanthophyllsFEBS Letters, 1996
- Examination of the possible involvement of lipoxygenase and jasmonates in pollination-induced senescence of Phalaenopsis and Dendrobium orchid flowersPhysiologia Plantarum, 1995
- The physiology of petal senescence which is not initiated by ethylenePublished by Cambridge University Press (CUP) ,1994
- Flower senescence in daylily (Hemerocallis)Physiologia Plantarum, 1992