Genetic Relatedness of Coagulase-negative Staphylococci From Gastrointestinal Tract and Blood of Preterm Neonates With Late-onset Sepsis
- 1 April 2013
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in The Pediatric Infectious Disease Journal
- Vol. 32 (4), 389-393
- https://doi.org/10.1097/inf.0b013e3182791abd
Abstract
Background: Coagulase-negative staphylococci (CoNS) are the first colonizers of gastrointestinal tract (GIT) and the commonest cause of late-onset sepsis (LOS) in preterm neonates. Intravascular catheters are considered a major source of CoNS bacteremia. However, several cases of LOS remain without an identified source. To elucidate whether GIT could be a potential source of invasive strains, we aimed to assess the molecular similarity between CoNS from blood and GIT in preterm neonates with LOS. Methods: Altogether 22 blood and 53 GIT isolates collected from 22 neonates with LOS caused by CoNS (Staphylococcus haemolyticus in 13, Staphylococcus epidermidis in 7 and Staphylococcus hominis in 2 patients) were included. Rectal swabs were collected twice weekly from birth, but only isolates obtained before LOS were analyzed. S. epidermidis isolates were typed by multilocus variable number of tandem repeats analysis and multilocus sequence typing, S. haemolyticus by pulsed-field gel electrophoresis. Results: Eighteen of 22 neonates had the same CoNS species in GIT and bloodstream; all these isolates from them (altogether 18 blood and 28 GIT isolates) underwent typing. The genotypic similarity between bloodstream and ≥1 antecedent GIT isolates was observed in 13 of 18 patients—3 of 7 with S. epidermidis and 10 of 11 with S. haemolyticus infection. The concordant GIT isolates were collected 0–7 days before the positive blood culture. Conclusions: The similarity between CoNS from GIT and bloodstream indicates that preterm neonates harbour invasive strains in GIT before LOS. Whether there is a causal relationship between GIT colonization and LOS remains to be elucidated in further studies.Keywords
This publication has 22 references indexed in Scilit:
- Mucosal surveillance cultures in predicting Gram-negative late-onset sepsis in neonatal intensive care unitsJournal of Hospital Infection, 2011
- Concordance of Gastrointestinal Tract Colonization and Subsequent Bloodstream Infections With Gram-negative Bacilli in Very Low Birth Weight Infants in the Neonatal Intensive Care UnitThe Pediatric Infectious Disease Journal, 2010
- Impact of empiric antibiotic regimen on bowel colonization in neonates with suspected early onset sepsisEuropean Journal of Clinical Microbiology & Infectious Diseases, 2010
- Comparison of ampicillin plus gentamicin vs. penicillin plus gentamicin in empiric treatment of neonates at risk of early onset sepsisActa Paediatrica, 2010
- Assessment of the bacterial diversity of human colostrum and screening of staphylococcal and enterococcal populations for potential virulence factorsResearch in Microbiology, 2008
- Minimal enteral feeding reduces the risk of sepsis in feed‐intolerant very low birth weight newbornsActa Paediatrica, 2008
- Staphylococcus epidermidis: A differential trait of the fecal microbiota of breast-fed infantsBMC Microbiology, 2008
- Cohort Study of the Pathogenesis and Molecular Epidemiology of Catheter-Related Bloodstream Infection in Neonates With Peripherally Inserted Central Venous CathetersInfection Control & Hospital Epidemiology, 2008
- THE GASTROINTESTINAL TRACT SERVES AS THE RESERVOIR FOR GRAM-NEGATIVE PATHOGENS IN VERY LOW BIRTH WEIGHT INFANTSThe Pediatric Infectious Disease Journal, 2007
- Improved Multilocus Sequence Typing Scheme for Staphylococcus epidermidisJournal of Clinical Microbiology, 2007