Influence of surface charge and transmembrane potential on rubidium-86 efflux of human red blood cells
- 1 January 1984
- journal article
- research article
- Published by Springer Science and Business Media LLC in The Journal of Membrane Biology
- Vol. 78 (3), 249-255
- https://doi.org/10.1007/bf01925972
Abstract
The dependence of the rate constant of Rb+ efflux on extracellular cation concentration was measured. At low ionic strengths Rb+ efflux increased strongly. Permeability coefficients were calculated from the rate constants measured, using the Goldman flux equation, with and without making allowance for surface potentials. Only when allowance was made for surface potentials and the associated differences beween ion concentrations in the bulk solutions and at the membrane surface, the permeability coefficient remained constant. Best agreement between experimental data and theoretically calculated values was obtained when an interior surface potential of − 110 mV was assumed. When the surface charge of erythrocytes is reduced by neuraminidase, the rate constants for Rb+ efflux decreased, indicating a significant influence of surface potential.This publication has 21 references indexed in Scilit:
- Echinocyte formation induced by potential changes of human red blood cellsThe Journal of Membrane Biology, 1982
- 309 - Electrostatic and structural properties of the surface of human erythrocytes. I-cell-electrophoretic studies following neuraminidase treatmentBioelectrochemistry and Bioenergetics, 1980
- 288 - Electrophoretical study of cell surface properties. The influence of the surface coat on the electric potential distribution and on general electrokinetic properties of animal cellsBioelectrochemistry and Bioenergetics, 1979
- The shape of red blood cells as a function of membrane potential and temperatureThe Journal of Membrane Biology, 1979
- 278 - Osmotic states of the red blood cellsBioelectrochemistry and Bioenergetics, 1979
- The Red Cell MembraneAnnual Review of Biochemistry, 1976
- The biological membrane potential some theoretical considerationsJournal of Theoretical Biology, 1976
- The cation permeability of erythrocytes in low ionic strength media of various tonicitiesThe Journal of Membrane Biology, 1969
- POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANESThe Journal of general physiology, 1943
- Die Ionenpermeabilität der Erythrocyten in NichtleiterlösungenPflügers Archiv - European Journal of Physiology, 1940