Msx and Dlx Homeogene Expression in Epithelial Odontogenic Tumors
Open Access
- 15 September 2008
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 57 (1), 69-78
- https://doi.org/10.1369/jhc.2008.951707
Abstract
Epithelial odontogenic tumors are rare jaw pathologies that raise clinical diagnosis and prognosis dilemmas notably between ameloblastomas and clear cell odontogenic carcinomas (CCOCs). In line with previous studies, the molecular determinants of tooth development—amelogenin, Msx1, Msx2, Dlx2, Dlx3, Bmp2, and Bmp4—were analyzed by RT-PCR, ISH, and immunolabeling in 12 recurrent ameloblastomas and in one case of CCOC. Although Msx1 expression imitates normal cell differentiation in these tumors, other genes showed a distinct pattern depending on the type of tumor and the tissue involved. In benign ameloblastomas, ISH localized Dlx3 transcripts and inconstantly detected Msx2 transcripts in epithelial cells. In the CCOC, ISH established a lack of both Dlx3 and Msx2 transcripts but allowed identification of the antisense transcript of Msx1, which imitates the same scheme of distribution between mesenchyme and epithelium as in the cup stage of tooth development. Furthermore, while exploring the expression pattern of signal molecules by RT-PCR, Bmp2 was shown to be completely inactivated in the CCOC and irregularly noticeable in ameloblastomas. Bmp4 was always expressed in all the tumors. Based on the established roles of Msx and Dlx transcription factors in dental cell fates, these data suggest that their altered expression is a proposed trail to explain the genesis and/or the progression of odontogenic tumors.Keywords
This publication has 77 references indexed in Scilit:
- Ameloblastic carcinoma: An analysis of 14 casesOral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology, 2007
- A Genome-Wide Screen for Promoter Methylation in Lung Cancer Identifies Novel Methylation Markers for Multiple MalignanciesPLoS Medicine, 2006
- Transforming potential of the T-cell acute lymphoblastic leukemia-associated homeobox genesHOXA13,TLX1, andTLX3Genes, Chromosomes and Cancer, 2006
- A case of amelogenesis imperfecta, cleft lip and palate and polycystic kidney diseaseOrthodontics & Craniofacial Research, 2006
- Expression pattern of Dlx3 during cell differentiation in mineralized tissuesBone, 2005
- DLX3 mutation associated with autosomal dominant amelogenesis imperfecta with taurodontismAmerican Journal of Medical Genetics Part A, 2005
- HOXandNon‐HOXHomeobox Genes in Leukemic HematopoiesisThe International Journal of Cell Cloning, 2002
- Bone Morphogenetic Protein-2 (BMP-2) Signaling to the Col2α1 Gene in Chondroblasts Requires the Homeobox Gene Dlx-2DNA and Cell Biology, 2001
- Msx1 Gene Overexpression Induces G1 Phase Cell Arrest in Human Ovarian Cancer Cell Line OVCAR3Biochemical and Biophysical Research Communications, 2001
- Identification of BMP-4 as a signal mediating secondary induction between epithelial and mesenchymal tissues during early tooth developmentCell, 1993