Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling
- 15 January 2009
- journal article
- Published by The Company of Biologists in Development
- Vol. 136 (2), 327-335
- https://doi.org/10.1242/dev.025742
Abstract
One striking feature of dentate gyrus development, distinct from the other cortical structures, is the relocation of neural precursors from the ventricular zone to the forming dentate pole to produce a lifelong neurogenic subgranular zone (SGZ). In this study, we demonstrate that dentate progenitors first dwell for up to 1 week in a previously unrecognized neurogenic zone intimately associated with the pial meningeal surface lining the outer edge of the forming dentate. This zone also serves as the organizational matrix for the initial formation of the dentate glial scaffolding. Timely clearance of neural precursors from their transient location depends on reelin, whereas initial formation of this transient stem cell niche requires Cxcl12-Cxcr4 signaling. The final settlement of the neural precursors at the subgranular zone relies on a pertussis toxin-sensitive pathway independent of Cxcl12-Cxcr4 signaling. Furthermore, genetic fate-mapping analysis suggests that subpial precursors contribute to the SGZ formation. These results demonstrate that the relocation of neural precursors in the dentate gyrus consists of discrete steps regulated by multiple pathways.Keywords
This publication has 38 references indexed in Scilit:
- Tbr2 Directs Conversion of Radial Glia into Basal Precursors and Guides Neuronal Amplification by Indirect Neurogenesis in the Developing NeocortexNeuron, 2008
- The T-box transcription factor Eomes/Tbr2 regulates neurogenesis in the cortical subventricular zoneGenes & Development, 2008
- Regional Distribution of Cortical Interneurons and Development of Inhibitory Tone Are Regulated by Cxcl12/Cxcr4 SignalingJournal of Neuroscience, 2008
- Probing cell type–specific functions of Gi in vivo identifies GPCR regulators of insulin secretionJCI Insight, 2007
- Chemokine receptor expression by neural progenitor cells in neurogenic regions of mouse brainJournal of Comparative Neurology, 2006
- Stromal-Derived Factor-1 (CXCL12) Regulates Laminar Position of Cajal-Retzius Cells in Normal and Dysplastic BrainsJournal of Neuroscience, 2006
- Endothelial Cells Stimulate Self-Renewal and Expand Neurogenesis of Neural Stem CellsScience, 2004
- Wnt Signaling Mutants Have Decreased Dentate Granule Cell Production and Radial Glial Scaffolding AbnormalitiesJournal of Neuroscience, 2004
- Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivoNature Neuroscience, 2002
- Mammalian Neural Stem CellsScience, 2000