Cell-Based Screen for Identification of Inhibitors of Tubulin Polymerization
- 1 January 1996
- journal article
- Published by American Chemical Society (ACS) in Journal of Natural Products
- Vol. 59 (12), 1179-1182
- https://doi.org/10.1021/np960144k
Abstract
This assay is based on morphological changes of rat glioma cells treated with db-cAMP. The db-cAMP treatment induces a tubulin-dependent change causing the cells to acquire a spherical shape. Pretreatment with tubulin inhibitors brings about the disintegration of tubulin polymer and/or prevents its polymerization. Cells with inhibited tubulin fail to respond to db-cAMP treatment. Cells treated with inhibitors of tubulin polymerization are then separated from the spherical cells by aspiration. A semiautomated scanning procedure evaluates the final culture density and yields graphical data.Keywords
This publication has 17 references indexed in Scilit:
- Structure of Curacin A, a Novel Antimitotic, Antiproliferative and Brine Shrimp Toxic Natural Product from the Marine Cyanobacterium Lyngbya majusculaThe Journal of Organic Chemistry, 1994
- Effect of dibutyryl cyclic amp on morphologic features and marker production of human cervical argyrophil small-cell carcinoma cell lineInternational Journal of Cancer, 1994
- A rapid mechanism-based screen to detect potential anti-cancer agentsAnti-Cancer Drugs, 1994
- Effect of Dibutyryl Cyclic Adenosine 3',5'-Monophosphate on Morphological Features and Biological Markers of Human Salivary Myoepithelial Cell Line in CultureCancer Investigation, 1992
- Stabilization of actin filaments at early times after adenovirus infection and in heat-shocked cellsVirus Research, 1991
- A Rapid and Sensitive Bioassay Involving Cultured Rat Glioma Cells to Screen for Substances Capable of Elevating Intracellular Cyclic AMP ConcentrationJournal of Natural Products, 1988
- Current Status of the NCI Plant and Animal Product ProgramJournal of Natural Products, 1982
- Inhibition of tubulin polymerization by MebendazoleBiochemical and Biophysical Research Communications, 1980
- Morphological Changes in Rat Glioma Cells caused by Adenosine Cyclic 3', 5'-MonophosphateCell Structure and Function, 1978
- Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubulesJournal of Molecular Biology, 1974