Unipept 4.0: Functional Analysis of Metaproteome Data
- 22 November 2018
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 18 (2), 606-615
- https://doi.org/10.1021/acs.jproteome.8b00716
Abstract
Unipept (https://unipept.ugent.be) is a web application for metaproteome data analysis, with an initial focus on tryptic peptide based biodiversity analysis of MS/MS samples. As the true potential of metaproteomics lies in gaining insights in the expressed functions of complex environmental samples, the 4.0 release of Unipept introduces complementary functional analysis based on GO terms and EC numbers. Integration of this new functional analysis with the existing biodiversity analysis is an important asset of the extended pipeline. As a proof of concept, a human faecal metaproteome dataset from 15 healthy subjects was reanalysed with Unipept 4.0, yielding fast, detailed and straightforward characterization of taxon-specific catalytic functions that is shown to be consistent with earlier results from a BLAST-based functional analysis of the same data.Keywords
Funding Information
- Fonds Wetenschappelijk Onderzoek (12I5217N, G042518N)
- ELIXIR Belgium
This publication has 35 references indexed in Scilit:
- Intestinal Metaproteomics Reveals Host-Microbiota Interactions in Subjects at Risk for Type 1 DiabetesDiabetes Care, 2018
- Metaproteomics reveals associations between microbiome and intestinal extracellular vesicle proteins in pediatric inflammatory bowel diseaseNature Communications, 2018
- Comparative metaproteomics analysis shows altered fecal microbiota signatures in patients with major depressive disorderNeuroReport, 2018
- Ecological and functional adaptations to water management in a semiarid agroecosystem: a soil metaproteomics approachScientific Reports, 2017
- Metaproteomics as a Complementary Approach to Gut Microbiota in Health and DiseaseFrontiers in Chemistry, 2017
- Unravelling the effect of clostridia spores and lysozyme on microbiota dynamics in Grana Padano cheese: A metaproteomics approachJournal of Proteomics, 2016
- Faecal proteomics: A tool to investigate dysbiosis and inflammation in patients with cystic fibrosisJournal of Cystic Fibrosis, 2015
- Microbial metaproteomics for characterizing the range of metabolic functions and activities of human gut microbiotaProteomics, 2015
- Shotgun metaproteomics of the human distal gut microbiotaThe ISME Journal, 2008
- Metaproteomics Provides Functional Insight into Activated Sludge Wastewater TreatmentPLOS ONE, 2008