Resonance Raman spectroscopy
- 1 July 2009
- journal article
- review article
- Published by Springer Science and Business Media LLC in Photosynthesis Research
- Vol. 101 (2-3), 147-155
- https://doi.org/10.1007/s11120-009-9440-4
Abstract
Resonance Raman spectroscopy may yield precise information on the conformation of, and on the interactions assumed by, the chromophores involved in the first steps of the photosynthetic process, whether isolated in solvents, embedded in soluble or membrane proteins, or, as shown recently, in vivo. By making use of this technique, it is possible, for instance, to relate the electronic properties of these molecules to their structure and/or the physical properties of their environment, or to determine subtle changes of their conformation associated with regulatory processes. After a short introduction to the physical principles that govern resonance Raman spectroscopy, the information content of resonance Raman spectra of chlorophyll and carotenoid molecules is described in this review, together with the experiments which helped in determining which structural parameter each Raman band is sensitive to. A selection of applications of this technique is then presented, in order to give a fair and precise idea of which type of information can be obtained from its use in the field of photosynthesis.Keywords
This publication has 43 references indexed in Scilit:
- Two Stereoisomers of Spheroidene in the Rhodobacter sphaeroides R26 Reaction Center: A DFT Analysis of Resonance Raman SpectraBiophysical Journal, 2007
- Crystal structure of spinach major light-harvesting complex at 2.72 Å resolutionNature, 2004
- Non-bonding molecular factors influencing the stretching wavenumbers of the conjugated carbonyl groups of bacteriochlorophyllaJournal of Raman Spectroscopy, 1998
- Effects of vinyl substitutions on resonance Raman spectra of (bacterio)chlorophyllsJournal of Raman Spectroscopy, 1994
- Application of near-IR Fourier transform resonance Raman spectroscopy to the study of photosynthetic proteinsSpectrochimica Acta Part A: Molecular Spectroscopy, 1993
- Genetically modified photosynthetic antenna complexes with blueshifted absorbance bandsNature, 1992
- Primary donor structure and interactions in bacterial reaction centers from near-infrared Fourier transform resonance Raman spectroscopyBiochemistry, 1991
- Raman and infrared spectra of the all‐trans, 7‐cis, 9‐cis, 13‐cis and 15‐cis isomers of β‐carotene: Key bands distinguishing stretched or terminal‐bent configurations form central‐bent configurationsJournal of Raman Spectroscopy, 1988
- Structures of antenna complexes of several Rhodospirillales from their resonance Raman spectraBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Vibrational spectra of some carotenoids and related linear polyenes. Raman spectroscopic studyJournal of the American Chemical Society, 1973