Capillary zone electrophoresis of reducing mono- and oligo-saccharides as the borate complexes of their 3-methyl-1-phenyl-2-pyrazolin-5-one derivatives
- 13 August 1991
- journal article
- Published by Elsevier BV in Carbohydrate Research
- Vol. 215 (1), 193-198
- https://doi.org/10.1016/0008-6215(91)84018-a
Abstract
Various aldoses were derivatized to their 3-methyl-1-phenyl-2-pyrazolin-5-one derivatives and analyzed by capillary zone electrophoresis as their borate complexes. All derivatives of aldopentoses and aldohexoses were completely separated from each other in 100mm borate buffer (pH 9.5) and detected by u.v. absorption in the on-column mode. This system also provided good separation of derivatives of homologous oligoglucans having various types of interglycosidic linkages.Keywords
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