Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry
- 6 March 2003
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 17 (8), 845-850
- https://doi.org/10.1002/rcm.984
Abstract
The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT‐MS). This technique enables on‐line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK‐MES and CALU‐1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37°C and found to be proportional to the number of cancer cells in the medium (typically 108). From these data, the acetaldehyde production rates of the SK‐MES cells and the CALU‐1 cells in vitro are determined to be 1 × 106 and 1.5–3 × 106 molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed. Copyright © 2003 John Wiley & Sons, Ltd.Keywords
Funding Information
- Engineering and Physical Sciences Research Council, UK (GR/M89195/01)
- Grant Agency of the Czech Republic
This publication has 34 references indexed in Scilit:
- Time variation of ammonia, acetone, isoprene and ethanol in breath: a quantitative SIFT-MS study over 30 daysPhysiological Measurement, 2003
- Quantification of volatile compounds in the headspace of aqueous liquids using selected ion flow tube mass spectrometryRapid Communications in Mass Spectrometry, 2002
- Relationship between acetaldehyde levels and cell survival in ethanol-metabolizing hepatoma cellsHepatology, 2002
- Kinetics and isotope patterns of ethanol and acetaldehyde emissions from yeast fermentations of glucose and glucose‐6,6‐d2 using selected ion flow tube mass spectrometry: a case studyRapid Communications in Mass Spectrometry, 2001
- A new ‘online’ method to measure increased exhaled isoprene in end‐stage renal failureNephrology Dialysis Transplantation, 2001
- A Factor from Pancreatic and Colonic Cancer Cells Stimulates Glucose Uptake and Lactate Production in MyoblastsBiochemical and Biophysical Research Communications, 1999
- Cancer statistics, 1999CA: A Cancer Journal for Clinicians, 1999
- Application of ion chemistry and the SIFT technique to the quantitative analysis of trace gases in air and on breathInternational Reviews in Physical Chemistry, 1996
- Mechanisms of Acetaldehyde-Mediated Growth Inhibition: Delayed Cell Cycle Progression and Induction of ApoptosisAlcohol: Clinical and Experimental Research, 1995
- Expired Hydrocarbons in Patients with Acute Myocardial InfarctionFree Radical Research, 1995