Seed-specific promoters direct gene expression in non-seed tissue
Open Access
- 4 June 2004
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 55 (402), 1463-1471
- https://doi.org/10.1093/jxb/erh158
Abstract
The organ specificity of four promoters that are known to direct seed-specific gene expression was tested. Whereas the phaseolin (phas)- and legumin B4 (leB4)-promoters were from genes encoding 7S and 11S globulins from Phaseolus vulgaris and Vicia faba, respectively, the usp- and the sbp-promoters were from non-storage protein genes of V. faba. The expression of different promoter–reporter gene fusions was followed either by RT-PCR or by registering the reporter enzyme activity in organs of transgenic tobacco, pea, narbon bean, or linseed. In addition to seeds, the promoters directed reporter gene expression in pollen and in seed coats. USP-, vicilin- and legumin-mRNA were detected by RT-PCR in pollen of Pisum sativum and V. faba. Expression during microsporogenesis and embryogenesis seems to be a general character of various seed protein genes.Keywords
This publication has 29 references indexed in Scilit:
- The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue-specific expression of the SBP2 promoterJournal of Experimental Botany, 2003
- High-level and stable accumulation of single-chain Fv antibodies in plant storage organsJournal of Plant Physiology, 1998
- Purification, cDNA Cloning and Characterization of Proteinase B, an Asparagine-specific Endopeptidase from Germinating Vetch (Vicia sativa L.) SeedsEuropean Journal of Biochemistry, 1995
- A novel seed protein gene from Vicia faba is developmentally regulated in transgenic tobacco and Arabidopsis plantsMolecular Genetics and Genomics, 1991
- Upstream sequences regulating legumin gene expression in heterologous transgenic plantsMolecular Genetics and Genomics, 1991
- Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plantsPlant Molecular Biology, 1989
- Abundant embryonic mRNA in field bean (Vicia faba L.) codes for a new class of seed proteins: cDNA cloning and characterization of the primary translation productPlant Molecular Biology, 1988
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987
- The legumin gene family: structure of a B type gene ofVicia fabaand a possible legumin gene specific regulatory elementNucleic Acids Research, 1986
- Analysis of in vivo and in vitro Globulin Formation during Cotyledon Development of Field Beans (V icia faba L. var. minor)Biochemie und Physiologie der Pflanzen, 1983