The design and application of fluorophore–gold nanoparticle activatable probes
- 7 March 2011
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Physical Chemistry Chemical Physics
- Vol. 13 (21), 9929-9941
- https://doi.org/10.1039/c0cp02967j
Abstract
Fluorescence-based assays and detection techniques are among the most highly sensitive and popular biological tests for researchers. To match the needs of research and the clinic, detection limits and specificities need to improve, however. One mechanism is to decrease non-specific background signals, which is most efficiently done by increasing fluorescence quenching abilities. Reports in the literature of theoretical and experimental work have shown that metallic gold surfaces and nanoparticles are ultra-efficient fluorescence quenchers. Based on these findings, subsequent reports have described gold nanoparticle fluorescence-based activatable probes that were designed to increase fluorescence intensity based on a range of stimuli. In this way, these probes can detect and signify assorted biomarkers and changes in environmental conditions. In this review, we explore the various factors and theoretical models that affect gold nanoparticle fluorescence quenching, explore current uses of activatable probes, and propose an engineering approach for future development of fluorescence based gold nanoparticle activatable probes.Keywords
This publication has 74 references indexed in Scilit:
- Hairpin DNA-Functionalized Gold Colloids for the Imaging of mRNA in Live CellsJournal of the American Chemical Society, 2010
- Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytesProceedings of the National Academy of Sciences of the United States of America, 2010
- Plasmon-Enhanced Fluorescence from Single Fluorophores End-Linked to Gold NanorodsJournal of the American Chemical Society, 2010
- Matrix Metalloproteinases: Regulators of the Tumor MicroenvironmentCell, 2010
- Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent proteinNature Chemistry, 2009
- Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arraysProceedings of the National Academy of Sciences of the United States of America, 2009
- Molecular Engineering of DNA: Molecular BeaconsAngewandte Chemie-International Edition, 2009
- Cytokines in the pathogenesis of rheumatoid arthritisNature Reviews Immunology, 2007
- Photoluminescence Quenching of Conjugated Macromolecules by Bipyridinium Derivatives in Aqueous Media: Charge DependenceLangmuir, 2001
- Molecular Beacons: Probes that Fluoresce upon HybridizationNature Biotechnology, 1996