Analysis of Human Colon by Raman Spectroscopy and Imaging-Elucidation of Biochemical Changes in Carcinogenesis
Open Access
- 10 July 2019
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (14), 3398
- https://doi.org/10.3390/ijms20143398
Abstract
Noninvasive Raman imaging of non-fixed and unstained human colon tissues based on vibrational properties of noncancerous and cancerous samples can effectively enable the differentiation between noncancerous and tumor tissues. This work aimed to evaluate the biochemical characteristics of colon cancer and the clinical merits of multivariate Raman image and spectroscopy analysis. Tissue samples were collected during routine surgery. The non-fixed, fresh samples were used to prepare micrometer sections from the tumor mass and the tissue from the safety margins outside of the tumor mass. Adjacent sections were used for typical histological analysis. We have found that the chemical composition identified by Raman spectroscopy of the cancerous and the noncancerous colon samples is sufficiently different to distinguish pathologically changed tissue from noncancerous tissue. We present a detailed analysis of Raman spectra for the human noncancerous and cancerous colon tissue. The multivariate analysis of the intensities of lipids/proteins/carotenoids Raman peaks shows that these classes of compounds can statistically divide analyzed samples into noncancerous and pathological groups, reaffirming that Raman imaging is a powerful technique for the histochemical analysis of human tissues. Raman biomarkers based on ratios for lipids/proteins/carotenoids content were found to be the most useful biomarkers in spectroscopic diagnostics.Funding Information
- Narodowe Centrum Nauki (UMO-2017/25/B/ST4/01788)
This publication has 51 references indexed in Scilit:
- Multi-modal approach using Raman spectroscopy and optical coherence tomography for the discrimination of colonic adenocarcinoma from normal colonBiomedical Optics Express, 2013
- Gastric cancer detection based on blood plasma surface-enhanced Raman spectroscopy excited by polarized laser lightBiosensors and Bioelectronics, 2011
- Effect of beta carotene on lipid peroxidation and antioxidant status following renal ischemia/reperfusion injury in ratScandinavian Journal of Clinical and Laboratory Investigation, 2010
- Analysis of urinary nucleosides as potential tumor markers in human colorectal cancer by high performance liquid chromatography/electrospray ionization tandem mass spectrometryClinica Chimica Acta; International Journal of Clinical Chemistry, 2009
- HDACs, histone deacetylation and gene transcription: from molecular biology to cancer therapeuticsCell Research, 2007
- Raman spectroscopy: elucidation of biochemical changes in carcinogenesis of oesophagusBritish Journal of Cancer, 2006
- The Repression of E2F-1 Is Critical for the Activity of Minerval against CancerThe Journal of pharmacology and experimental therapeutics, 2005
- SERRS as a more sensitive technique for the detection of labelled oligonucleotides compared to fluorescenceThe Analyst, 2004
- Near‐infrared Raman spectroscopy for optical diagnosis of lung cancerInternational Journal of Cancer, 2003
- Temperature-dependent Raman and infrared spectra of nucleosides. II?CytidineJournal of Raman Spectroscopy, 2001