Perturbation of Host Cell Cytoskeleton by Cranberry Proanthocyanidins and Their Effect on Enteric Infections
Open Access
- 4 November 2011
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 6 (11), e27267
- https://doi.org/10.1371/journal.pone.0027267
Abstract
Cranberry-derived compounds, including a fraction known as proanthocyanidins (PACs) exhibit anti-microbial, anti-infective, and anti-adhesive properties against a number of disease-causing organisms. In this study, the effect of cranberry proanthocyanidins (CPACs) on the infection of epithelial cells by two enteric bacterial pathogens, enteropathogenic Escherichia coli (EPEC) and Salmonella Typhimurium was investigated. Immunofluorescence data showed that actin pedestal formation, required for infection by enteropathogenic Escherichia coli (EPEC), was disrupted in the presence of CPACs. In addition, invasion of HeLa cells by Salmonella Typhimurium was significantly reduced, as verified by gentamicin protection assay and immunofluorescence. CPACs had no effect on bacterial growth, nor any detectable effect on the production of bacterial effector proteins of the type III secretion system. Furthermore, CPACs did not affect the viability of host cells. Interestingly, we found that CPACs had a potent and dose-dependent effect on the host cell cytoskeleton that was evident even in uninfected cells. CPACs inhibited the phagocytosis of inert particles by a macrophage cell line, providing further evidence that actin-mediated host cell functions are disrupted in the presence of cranberry CPACs. Thus, although CPAC treatment inhibited Salmonella invasion and EPEC pedestal formation, our results suggest that this is likely primarily because of the perturbation of the host cell cytoskeleton by CPACs rather than an effect on bacterial virulence itself. These findings have significant implications for the interpretation of experiments on the effects of CPACs on bacteria-host cell interactions.Keywords
This publication has 46 references indexed in Scilit:
- Inhibition of Escherichia coli CFT073 fliC Expression and Motility by Cranberry MaterialsApplied and Environmental Microbiology, 2011
- The Swarming Motility of Pseudomonas aeruginosa Is Blocked by Cranberry Proanthocyanidins and Other Tannin-Containing MaterialsApplied and Environmental Microbiology, 2011
- Induction of a State of Iron Limitation in Uropathogenic Escherichia coli CFT073 by Cranberry-Derived Proanthocyanidins as Revealed by Microarray AnalysisApplied and Environmental Microbiology, 2011
- Salmonella takes control: effector-driven manipulation of the hostCurrent Opinion in Microbiology, 2009
- Antibacterial effects of American cranberry (Vaccinium macrocarpon) concentrate on foodborne pathogensLWT, 2008
- Cranberry Phytochemical Extract Inhibits SGC-7901 Cell Growth and Human Tumor Xenografts in Balb/c nu/nu MiceJournal of Agricultural and Food Chemistry, 2008
- Anticancer activities of cranberry phytochemicals: An updateMolecular Nutrition & Food Research, 2008
- Iron-binding properties of plant phenolics and cranberry's bio-effectsDalton Transactions, 2007
- Bioactive compounds in cranberries and their role in prevention of urinary tract infectionsMolecular Nutrition & Food Research, 2007
- Inhibition of the Adherence of P-FimbriatedEscherichia colito Uroepithelial-Cell Surfaces by Proanthocyanidin Extracts from CranberriesNew England Journal of Medicine, 1998