Comparison of Nucleolar Proteins of Normal Rat Liver and Novikoff Hepatoma Ascites Cells by Two-Dimensional Polyacrylamide Gel Electrophoresis
- 1 May 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 70 (5), 1316-1320
- https://doi.org/10.1073/pnas.70.5.1316
Abstract
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.Keywords
This publication has 7 references indexed in Scilit:
- Comparative high-resolution electrophoresis of tumor histones: variation in phosphorylation as a function of cell replication rate.1972
- Determination of the Number of Proteins in Liver Ribosomes and Ribosomal Subunits by Two-Dimensional Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1972
- Enumeration of rabbit reticulocyte ribosomal proteinsJournal of Molecular Biology, 1971
- Isolation of an electrophoretically homogeneous nonhistone nucleolar proteinLife Sciences, 1971
- Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.1971
- Limited heterogeneity of the major nonhistone chromosomal proteinsBiochemistry, 1970
- Ribosomal proteins. VIIAnalytical Biochemistry, 1970