Inhibition of the Ubiquitin-Proteasome System Affects Influenza A Virus Infection at a Postfusion Step
- 15 September 2010
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 84 (18), 9625-9631
- https://doi.org/10.1128/jvi.01048-10
Abstract
We have demonstrated that influenza A virus (IAV) RNA synthesis depends on the ubiquitin-proteasome system. IAV replication was reduced both by proteasome inhibitors and in E36ts20 cells, which contain the thermolabile ubiquitin-activating enzyme E1. While virus entry was not affected in E36ts20 cells, the proteasome inhibitor MG132 retained viral particles in the cytoplasm. Addition-removal experiments of MG132 in combination with bafilomycin A1, a well-established inhibitor of IAV entry and fusion, showed that MG132 affected IAV infection at a postfusion step. This was confirmed by the lack of inhibition of IAV entry by proteasome inhibitors in a virus-like particle fusion assay.Keywords
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