The detection of Salmonella in skimmed milk powder enrichments using conventional methods and immunomagnetic separation
- 28 June 1995
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 20 (6), 361-364
- https://doi.org/10.1111/j.1472-765x.1995.tb01321.x
Abstract
Skimmed milk powders were spiked with one of three Salmonella serovars and incubated in buffered peptone water for 24 h. No false‐negative results were obtained by immunomagnetic separation (IMS), compared to seven for selenite cysteine, one for Müller‐Kauffmann tetrathionate and two for Rappaport‐Vassiliadis enrichment broths. Salmonella virchow was detected and enumerated during the pre‐enrichment incubation by IMS and indirect conductance techniques. The Salm. virchow cell number did not increase after 12 h incubation and remained at 3 times 106 cfu ml‐1. IMS was able to capture Salm. virchow cells at cell numbers ca 50 ml‐1 in the presence of a 1000 greater number of non‐salmonella cells.Keywords
This publication has 6 references indexed in Scilit:
- The application of magnetic separations in applied microbiologyJournal of Applied Bacteriology, 1995
- IMS: a new selective enrichment technique for detection of Salmonella in foodsInternational Journal of Food Microbiology, 1994
- Rapid and alternative methods for the detection of salmonellas in foodsJournal of Applied Bacteriology, 1993
- Immunomagnetic separation as an alternative to enrichment broths for Salmonella detectionLetters in Applied Microbiology, 1993
- Detection of Salmonella in animal protein by Rappaport-Vassiliadis broth using indirect impediometryInternational Journal of Food Microbiology, 1993
- The detection of Salmonella enteritidis and S. typhimurium using immunomagnetic separation and conductance microbiologyLetters in Applied Microbiology, 1992