Expression Analysis of the NLRP Gene Family Suggests a Role in Human Preimplantation Development
Open Access
- 23 July 2008
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 3 (7), e2755
- https://doi.org/10.1371/journal.pone.0002755
Abstract
The NLRP (Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing) family, also referred to as NALP family, is well known for its roles in apoptosis and inflammation. Several NLRPs have been indicated as being involved in reproduction as well. We studied, using the unique human gametes and embryo materials, the expression of the NLRP family in human gametes and preimplantation embryos at different developmental stages, and compared the expression levels between normal and abnormal embryos using real-time PCR. Among 14 members of the NLRP family, twelve were detected in human oocytes and preimplantation embryos, whereas seven were detected in spermatozoa. Eight NLRPs (NLRP4, 5, 8, 9, 11, 12, 13, and 14) showed a similar expression pattern: their expression levels were high in oocytes and then decreased progressively in embryos, resulting in a very low level in day 5 embryos. However, NLRP2 and NLRP7 showed a different expression pattern: their expression decreased from oocytes to the lowest level by day 3, but increased again by day 5. The expression levels of NLRP5, 9, and 12 were lower in day 1 abnormal embryos but higher in day3 and day5 arrested embryos, when compared with normal embryos at the same stages. NLRP7 was down-regulated in day 1 and day 5 abnormal embryos but over-expressed in day3 arrested embryos. According to our results, different NLRPs possibly work in a stage-dependent manner during human preimplantation development.Keywords
This publication has 25 references indexed in Scilit:
- Women heterozygous forNALP7/NLRP7 mutations are at risk for reproductive wastage: report of two novel mutationsHuman Mutation, 2007
- Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cellsFertility and Sterility, 2007
- Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitroBMC Developmental Biology, 2007
- The Inflammasome: First Line of the Immune Response to Cell StressCell, 2006
- Mutations in the testis-specific NALP14 gene in men suffering from spermatogenic failureHuman Reproduction, 2006
- MATER protein expression and intracellular localization throughout folliculogenesis and preimplantation embryo development in the bovineBMC Developmental Biology, 2006
- Exposure In Vivo to Silica or Lipopolysaccharide Produces Transient or Sustained Upregulation, Respectively, of PYPAF7 and MEFV Genes in Bronchoalveolar Lavage Cells in RatsJournal of Toxicology and Environmental Health, Part A, 2006
- Mutations in NALP7 cause recurrent hydatidiform moles and reproductive wastage in humansNature Genetics, 2006
- PYPAF3, a PYRIN-containing APAF-1-like Protein, Is a Feedback Regulator of Caspase-1-dependent Interleukin-1β SecretionPublished by Elsevier BV ,2005
- Bovine mater‐like NALP9 is an oocyte marker geneMolecular Reproduction and Development, 2005