Cytokinesis-block micronucleus cytome assay
Top Cited Papers
Open Access
- 3 May 2007
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 2 (5), 1084-1104
- https://doi.org/10.1038/nprot.2007.77
Abstract
The cytokinesis-block micronucleus cytome assay is a comprehensive system for measuring DNA damage, cytostasis and cytotoxicity. DNA damage events are scored specifically in once-divided binucleated (BN) cells and include (a) micronuclei (MNi), a biomarker of chromosome breakage and/or whole chromosome loss, (b) nucleoplasmic bridges (NPBs), a biomarker of DNA misrepair and/or telomere end-fusions, and (c) nuclear buds (NBUDs), a biomarker of elimination of amplified DNA and/or DNA repair complexes. Cytostatic effects are measured via the proportion of mono-, bi- and multinucleated cells and cytotoxicity via necrotic and/or apoptotic cell ratios. Further information regarding mechanisms leading to MNi, NPBs and NBUDs formation is obtained using centromere and/or telomere probes. The assay is being applied successfully for biomonitoring of in vivo genotoxin exposure, in vitro genotoxicity testing and in diverse research fields such as nutrigenomics and pharmacogenomics as well as a predictor of normal tissue and tumor radiation sensitivity and cancer risk. The procedure can take up to 5 days to complete.Keywords
This publication has 96 references indexed in Scilit:
- Folic acid deficiency increases chromosomal instability, chromosome 21 aneuploidy and sensitivity to radiation-induced micronucleiMutation Research - Reviews in Mutation Research, 2005
- Methylenetetrahydrofolate reductase C677T polymorphism does not alter folic acid deficiency-induced uracil incorporation into primary human lymphocyte DNA in vitroCarcinogenesis: Integrative Cancer Research, 2001
- A structural analysis of cytoskeleton components during the execution phase of apoptosisProtoplasma, 1997
- A more comprehensive application of the micronucleus technique for biomonitoring of genetic damage rates in human populations—experiences from the Chernobyl catastropheEnvironmental and Molecular Mutagenesis, 1997
- Micronucleus induction in cytokinesis-blocked mouse bone marrow cells in vitro following in vivo exposure to x-irradiation and cyclophosphamideEnvironmental and Molecular Mutagenesis, 1994
- The use of fluorescence in situ hybridization for the detection of aneugens in cytokinesis-blocked mouse splenocytesMutagenesis, 1993
- Conversion of excision‐repairable DNA lesions to micronuclei within one cell cycle in human lymphocytesEnvironmental and Molecular Mutagenesis, 1992
- Concurrent detection of gene mutations and chromosomal aberrations induced in vivo in somatic cellsMutagenesis, 1990
- Initiating carcinogen, triethylenemelamine, induces micronuclei in skin target cellsEnvironmental and Molecular Mutagenesis, 1989
- Radiation induction of micronuclei in human lymphocytesMutation Research - Reviews in Mutation Research, 1988