Transcriptional regulation of the plastocyanin and cytochrome C553genes from the cyanobacterium Anabaena species PCC 7937
- 1 June 1992
- journal article
- Published by Wiley in Molecular Microbiology
- Vol. 6 (11), 1507-1513
- https://doi.org/10.1111/j.1365-2958.1992.tb00871.x
Abstract
The effect of copper on the levels of plastocyanin (PC) and cytochrome c553 (cyt-c)-specific transcripts from Anabaena sp. PCC 7937 was investigated. The addition of copper resulted in a marked increase in PC mRNA levels, and a decrease in cyt c mRNA levels. Thus the functional exchange between PC and cyt c seems to be regulated at the mRNA level. The copper-dependent increase in PC and decrease in cyt c mRNA levels was abolished when chloramphenicol was added to the cells. This suggests that de novo synthesis of at least one trans-acting element is required to regulate PC and cyt c mRNA levels. Both PC and cyt c mRNA stability was found to be unaltered under varying Cu2+ regimes. This leads to the conclusion that expression of both genes is regulated at the level of initiation of transcription.Keywords
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