Study of microRNA in diabetic nephropathy: Isolation, quantification and biological function
- 24 February 2015
- journal article
- review article
- Published by Wiley in Nephrology
- Vol. 20 (3), 132-139
- https://doi.org/10.1111/nep.12374
Abstract
In recent years, several studies have reported dysregulation of microRNA expression in disease with a growing interest focussed on targeting microRNAs as a novel therapy for human disease. This is especially true in diabetic nephropathy where the expression of several microRNAs is dysregulated, contributing to the increased expression and accumulation of extracellular matrix proteins and increased pro-fibrotic signalling, ultimately resulting in renal fibrosis. The development of various techniques and microRNA reagents has enabled work to progress very rapidly in this area. In the present article, the authors describe the methods they have used that have enabled them to contribute to our current understanding of the role of microRNAs in diabetic nephropathy.Keywords
This publication has 17 references indexed in Scilit:
- The shaping and functional consequences of the microRNA landscape in breast cancerNature, 2013
- Mapping the Human miRNA Interactome by CLASH Reveals Frequent Noncanonical BindingCell, 2013
- DIANA miRPath v.2.0: investigating the combinatorial effect of microRNAs in pathwaysNucleic Acids Research, 2012
- Suppression of microRNA-29 Expression by TGF-β1 Promotes Collagen Expression and Renal FibrosisJournal of the American Society of Nephrology, 2012
- Diabetes Complications: The MicroRNA PerspectiveDiabetes, 2011
- miR-200a Prevents Renal Fibrogenesis Through Repression of TGF-β2 ExpressionDiabetes, 2010
- Comprehensive modeling of microRNA targets predicts functional non-conserved and non-canonical sitesGenome Biology, 2010
- E-Cadherin Expression Is Regulated by miR-192/215 by a Mechanism That Is Independent of the Profibrotic Effects of Transforming Growth Factor-βDiabetes, 2010
- The impact of microRNAs on protein outputNature, 2008
- Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extractionAnalytical Biochemistry, 1987