Interaction of an NF-kappa B-like factor with a site upstream of the c-myc promoter.
- 1 June 1990
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 87 (12), 4727-4731
- https://doi.org/10.1073/pnas.87.12.4727
Abstract
The c-myc protooncogene has been implicated in control of growth and differentiation of mammalian cells. For instance, growth arrest is often preceded by reduction in c-myc mRNA and gene transcription. To elucidate the mechanisms of control of c-myc gene transcription, we have begun to characterize the interaction of nuclear factors with the 719-base-pair (bp) c-myc regulatory domain, located 1139-421 bp upstream of the P1 start site of the mouse gene. Nuclear extracts from exponentially growing WEHI 231 murine B-lymphoma cells formed multiple complexes in mobility-shift assays. Changes in complex distribution were observed in growth-arrested WEHI 231 cells, and a major site of this interaction mapped to a 21-bp sequence that is similar to the sequences recognized by the NF-kappa B family of proteins. Binding of NF-kappa B-like factors was demonstrated by oligonucleotide competition. Induction of complex formation upon 70Z/3 pre-B- to B-cell differentiation, enhancement of binding by GTP, and detergent-induced release of inhibitor protein suggested that NF-kappa B itself is one member of the family that can bind. Transfection of thymidine kinase-chloramphenicol acetyltransferase constructs containing the 21-bp c-myc sequence into Jurkat cells demonstrated increased chloramphenicol acetyltransferase activity upon phorbol ester and phytohemagglutinin treatment. These results suggest the involvement of NF-kappa B-like factors in the regulation of c-myc transcription.Keywords
This publication has 28 references indexed in Scilit:
- NF-κB: A pleiotropic mediator of inducible and tissue-specific gene controlCell, 1989
- A muscle-specific enhancer is located at the 3' end of the myosin light-chain 1/3 gene locus.Journal of Bone and Joint Surgery, 1988
- IκB: a Specific Inhibitor of the NF-κB Transcription FactorScience, 1988
- The same inducible nuclear proteins regulates mitogen activation of both the interleukin-2 receptor-alpha gene and type 1 HIVCell, 1988
- Activation of the HIV-1 LTR by T Cell Mitogens and the Trans-Activator Protein of HTLV-IScience, 1987
- Developmental stage specificity of the lymphoid V(D)J recombination activity.Published by Cold Spring Harbor Laboratory ,1987
- An inducible transcription factor activates expression of human immunodeficiency virus in T cellsNature, 1987
- Multiple nuclear factors interact with the immunoglobulin enhancer sequencesCell, 1986
- Mutations downstream of the polyadenylation site of a Xenopus β-globin mRNA affect the position but not the efficiency of 3′ processingCell, 1986
- A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosomeCell, 1984