GP4 of porcine reproductive and respiratory syndrome virus contains a neutralizing epitope that is susceptible to immunoselection in vitro
- 7 January 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 155 (3), 371-378
- https://doi.org/10.1007/s00705-009-0582-7
Abstract
Glycoprotein 4 (GP4) of porcine reproductive and respiratory syndrome virus (PRRSV) contains a highly variable neutralizing epitope. The present study aimed to investigate whether this epitope is susceptible to immunoselection by antibodies in vitro. Cultivation of PRRSV in vitro in the continuous presence of neutralizing monoclonal antibodies (mAbs) directed against this epitope resulted in the selection of mAb-resistant PRRSV strains within five passages. Comparison of the GP4 amino acid (aa) sequence of the original PRRSV strain with the GP4 aa sequences of the mAb-resistant PRRSV strains revealed aa substitutions within this epitope. In conclusion, this study shows that the neutralizing epitope on GP4 is susceptible to immunoselection by antibodies in vitro.Keywords
This publication has 38 references indexed in Scilit:
- Antigenic differences among porcine circovirus type 2 strains, as demonstrated by the use of monoclonal antibodiesJournal of General Virology, 2008
- Patterns of sequence evolution at epitopes for host antibodies and cytotoxic T-lymphocytes in human immunodeficiency virus type 1Virus Research, 2006
- Adaptability costs in immune escape variants of vesicular stomatitis virusVirus Research, 2005
- The primary GP5 neutralization epitope of North American isolates of porcine reproductive and respiratory syndrome virusVeterinary Immunology and Immunopathology, 2004
- Effect of virus-specific antibodies on attachment, internalization and infection of porcine reproductive and respiratory syndrome virus in primary macrophagesVeterinary Immunology and Immunopathology, 2004
- Duration of Infection and Proportion of Pigs Persistently Infected with Porcine Reproductive and Respiratory Syndrome VirusJournal of Clinical Microbiology, 2003
- Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 EctodomainJournal of Virology, 2002
- Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV)Veterinary Microbiology, 1997
- Effect of specific antibodies on the cell-associated spread of pseudorabies virus in monolayers of different cell typesArchiv für die gesamte Virusforschung, 1995
- Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell lineArchiv für die gesamte Virusforschung, 1993