Comparative Antibody-Mediated Phagocytosis ofStaphylococcus epidermidisCells Grown in a Biofilm or in the Planktonic State
- 1 August 2006
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 74 (8), 4849-4855
- https://doi.org/10.1128/iai.00230-06
Abstract
Staphylococcus epidermidisis an important cause of nosocomial infections. Virulence is attributable to elaboration of biofilms on medical surfaces that protect the organisms from immune system clearance. Even though leukocytes can penetrate biofilms, they fail to phagocytose and kill bacteria. The properties that make biofilm bacteria resistant to the immune system are not well characterized. In order to better understand the mechanisms of resistance of bacteria in biofilms to the immune system, we evaluated antibody penetration throughout the biofilm and antibody-mediated phagocytic killing of planktonic versus biofilm cells ofS. epidermidisby using a rabbit antibody to poly-N-acetylglucosamine (PNAG). These antibodies are opsonic and protect against infection with planktonic cells of PNAG-positiveStaphylococcus aureusandS. epidermidis. Antibody to PNAG readily penetrated the biofilm and bound to the same areas in the biofilm as did wheat germ agglutinin, a lectin known to bind to components of staphylococcal biofilms. However, biofilm cells were more resistant to opsonic killing than their planktonic counterparts in spite of producing more PNAG per cell than planktonic cells. Biofilm extracts inhibited opsonic killing mediated by antibody to PNAG, suggesting that the PNAG antigen within the biofilm matrix prevents antibody binding close to the bacterial cell surface, which is needed for efficient opsonic killing. Increased resistance of biofilm cells to opsonic killing mediated by an otherwise protective antibody was due not to a biofilm-specific phenotype but rather to high levels of antigen within the biofilm that prevented bacterial opsonization by the antibody.Keywords
This publication has 39 references indexed in Scilit:
- Effects of Growth in the Presence of Subinhibitory Concentrations of Dicloxacillin on Staphylococcus epidermidis and Staphylococcus haemolyticus BiofilmsApplied and Environmental Microbiology, 2005
- Comparative Opsonic and Protective Activities of Staphylococcus aureus Conjugate Vaccines Containing Native or Deacetylated Staphylococcal Poly- N -Acetyl-β-(1-6)-GlucosamineInfection and Immunity, 2005
- Poly- N -Acetylglucosamine Production in Staphylococcus aureus Is Essential for Virulence in Murine Models of Systemic InfectionInfection and Immunity, 2005
- Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetryJournal of Antimicrobial Chemotherapy, 2005
- Use of Confocal Microscopy To Analyze the Rate of Vancomycin Penetration through Staphylococcus aureus BiofilmsAntimicrobial Agents and Chemotherapy, 2005
- Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune systemCellular Microbiology, 2004
- Immunochemical Properties of the Staphylococcal Poly- N -Acetylglucosamine Surface PolysaccharideInfection and Immunity, 2002
- Biofilms: Survival Mechanisms of Clinically Relevant MicroorganismsClinical Microbiology Reviews, 2002
- RecurrentStaphylococcus aureusBacteremia: Pulsed‐Field Gel Electrophoresis Findings in 29 PatientsThe Journal of Infectious Diseases, 1999
- Molecular basis of intercellular adhesion in the biofilm‐forming Staphylococcus epidermidisMolecular Microbiology, 1996