Isolation and Characterization of Four Microsatellite Loci in the Tidewater Goby (Eucyclogobius newberryi)

Abstract

The first microsatellite loci for the endangered tidewater goby Eucyclogobius newberryi are described. A partial tidewater goby genomic library was constructed in pUC19 plasmid. Microsatellite-containing clones were isolated, sequenced, and amplified. Out of 12 positive clones sequenced, 3 were polymorphic and proved useful in a preliminary genetic screen of 3 well-separated populations (50 individuals each) along the central California coast. The number of alleles per locus ranged from 2 to 3. This initial study suggests a population bottleneck had occurred in one of the populations.