Simultaneous analysis of coumarin derivatives in extracts of Radix Angelicae pubescentis (Duhuo) by HPLC-DAD-ESI-MSn technique
- 11 August 2014
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Analytical Methods
- Vol. 6 (19), 7996-8002
- https://doi.org/10.1039/c4ay01468e
Abstract
Radix Angelicae pubescentis (Duhuo) is a traditional Chinese herbal medicine (TCM) used for the treatment of rheumatic diseases. The coumarin derivatives in the Duhuo extracts are important bioactive compounds. In this work, a high performance liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MSn) method was established for the rapid separation and characterization of coumarin compounds from the Duhuo extracts. A total of 24 coumarin compounds were detected, of which 7 compounds were unambiguously characterized by comparing their retention time (tR), UV and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the positive ion mode on line. Importantly, five of these characterized coumarin compounds were reported from this plant for the first time.Keywords
This publication has 38 references indexed in Scilit:
- The pharmacokinetics and oral bioavailability studies of columbianetin in rats after oral and intravenous administrationJournal of Ethnopharmacology, 2013
- In vitro permeability analysis, pharmacokinetic and brain distribution study in mice of imperatorin, isoimperatorin and cnidilin in Radix Angelicae DahuricaeFitoterapia, 2013
- The Canadian medicinal plant Heracleum maximum contains antimycobacterial diynes and furanocoumarinsJournal of Ethnopharmacology, 2013
- Investigation of the biotransformation of osthole by liquid chromatography/tandem mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2013
- Simultaneous quantification of polymethoxylated flavones and coumarins in Fructus aurantii and Fructus aurantii immaturus using HPLC–ESI-MS/MSJournal of Pharmaceutical and Biomedical Analysis, 2012
- Major furocoumarins in grapefruit juice I: Levels and urinary metabolite(s)Food and Chemical Toxicology, 2011
- Anthelmintic activity of the crude extracts, fractions, and osthole from Radix angelicae pubescentis against Dactylogyrus intermedius in goldfish (Carassius auratus) in vivoZeitschrift für Parasitenkunde, 2010
- Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometryJournal of Chromatography A, 2009
- Quantitative Determination of the Chemical Profile of the Plant Material “Qiang-huo” by LC-ESI-MS-MSChromatographia, 2006
- Furocoumarins from grapefruit juice and their effect on human CYP 3A4 and CYP 1B1 isoenzymesBioorganic & Medicinal Chemistry, 2006