Isolation and Comparative Study of Cell‐Free Nucleic Acids from Human Urine
- 5 September 2006
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1075 (1), 334-340
- https://doi.org/10.1196/annals.1368.045
Abstract
Cell-free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150-400 bp represent the main part of cell-free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell-free DNA isolated from their urine by methylation-specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARbeta2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell-free urine DNA in cancer diagnostics.Keywords
This publication has 15 references indexed in Scilit:
- Tumor Suppressor Gene Promoter Hypermethylation in Serum of Breast Cancer PatientsClinical Cancer Research, 2004
- Transrenal DNA as a Diagnostic Tool: Important Technical NotesAnnals of the New York Academy of Sciences, 2004
- Cell‐Surface‐Bound Nucleic Acids: Free and Cell‐Surface‐Bound Nucleic Acids in Blood of Healthy Donors and Breast Cancer PatientsAnnals of the New York Academy of Sciences, 2004
- Human Urine Contains Small, 150 to 250 Nucleotide-Sized, Soluble DNA Derived from the Circulation and May Be Useful in the Detection of Colorectal CancerThe Journal of Molecular Diagnostics, 2004
- World patent information––the first 25 yearsWorld Patent Information, 2004
- Cell-free nucleic acids in plasma, serum and urine: a new tool in molecular diagnosisAnnals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2003
- Circulating nucleic acids in plasma and serum as a noninvasive investigation for cancer: Time for large‐scale clinical studies?International Journal of Cancer, 2002
- Stability of Endogenous and Added RNA in Blood Specimens, Serum, and PlasmaClinical Chemistry, 2002
- Evidence of epigenetic changes affecting the chromatin state of the retinoic acid receptor β2 promoter in breast cancer cellsOncogene, 2000
- Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proceedings of the National Academy of Sciences of the United States of America, 1996