Three‐dimensional, in vivo MRI with self‐gating and image coregistration in the mouse
Open Access
- 27 February 2009
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 61 (5), 1148-1157
- https://doi.org/10.1002/mrm.21945
Abstract
Motion during magnetic resonance imaging (MRI) scans routinely results in undesirable image artifact or blurring. Since high‐resolution, three‐dimensional (3D) imaging of the mouse requires long scan times for satisfactory signal‐to‐noise ratio (SNR) and image quality, motion‐related artifacts are likely over much of the body and limit applications of mouse MRI. In this investigation, we explored the use of self‐gated imaging methods and image coregistration for improving image quality in the presence of motion. Self‐gated signal results from a modified 3D gradient‐echo sequence showed detection of periodic respiratory and cardiac motion in the adult mouse—with excellent comparison to traditional measurements, sensitivity to respiration‐induced tissue changes in the brain, and even detection of embryonic cardiac motion in utero. Serial image coregistration with rapidly‐acquired, low‐SNR volumes further enabled detection and correction of bulk changes in embryo location during in utero imaging sessions and subsequent reconstruction of high‐quality images. These methods, in combination, are shown to expand the range of applications for 3D mouse MRI, enabling late‐stage embryonic heart imaging and introducing the possibility of longitudinal developmental studies from embryonic stages through adulthood. Magn Reson Med, 2009.Keywords
This publication has 47 references indexed in Scilit:
- Cortical thickness measured from MRI in the YAC128 mouse model of Huntington's diseaseNeuroImage, 2008
- Mn enhancement and respiratory gating for in utero MRI of the embryonic mouse central nervous systemMagnetic Resonance in Medicine, 2008
- Cardiac structure and function during ageing in energetically compromised Guanidinoacetate N-methyltransferase (GAMT)-knockout mice – a one year longitudinal MRI studyJournal of Cardiovascular Magnetic Resonance, 2008
- MRI in mouse developmental biologyNMR in Biomedicine, 2007
- Comparison between prospective and retrospective triggering for mouse cardiac MRINMR in Biomedicine, 2006
- Generalized self‐navigated motion detection technique: Preliminary investigation in abdominal imagingMagnetic Resonance in Medicine, 2006
- In utero time-course assessment of mouse embryo development using high resolution magnetic resonance imagingBrain Structure and Function, 2002
- Retrospective estimation and correction of physiological artifacts in fMRI by direct extraction of physiological activity from MR dataMagnetic Resonance in Medicine, 1996
- Orbital navigator echoes for motion measurements in magnetic resonance imagingMagnetic Resonance in Medicine, 1995
- Extraction of cardiac and respiratory motion cycles by use of projection data and its applications to nmr imagingMagnetic Resonance in Medicine, 1990