Improved procedures for immunoferritin labeling of ultrathin frozen sections.
Open Access
- 1 December 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 71 (3), 894-906
- https://doi.org/10.1083/jcb.71.3.894
Abstract
In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross-linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems.This publication has 9 references indexed in Scilit:
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