Characterization of an alkaline active – thiol forming extracellular serine keratinase by the newly isolated Bacillus pumilus
- 8 October 2007
- journal article
- Published by Oxford University Press (OUP) in Journal of Applied Microbiology
- Vol. 104 (2), 411-419
- https://doi.org/10.1111/j.1365-2672.2007.03564.x
Abstract
Aims: The aim of the study was to optimize microbial degradation of keratinous waste and to characterize the alkaline active keratinase showing its biotechnological importance. Method and Results: An extracellular keratinase enzyme was purified from the culture medium of a bacterial isolate and the conditions were optimized. The molecular weight of DEAE‐Sepharose‐purified keratinase was determined by SDS‐PAGE. Instrumental analyses were investigated to study the mechanism of bovine hair hydrolysis. Isolate was identified as Bacillus pumilus based on phenotypic characteristics and 16S rDNA sequence. The optimized condition for its growth was pH 8 and 35°C. The molecular weight of the keratinase was estimated as 65 kDa. Activity inhibition by phenyl methyl sulphonyl fluoride confirmed keratinase as serine protease type. Instrumental analysis revealed the sulphitolysis and proteolysis involved mechanism in bovine hair hydrolysis. Conclusion: This study indicates that the isolated keratinase is an alkaline active serine protease with a high degree of activity towards bovine hair. Significance and Impact of the Study: This study examines a serine protease with high keratinolytic activity and degradation mechanism for bovine hair. The keratinolytic activity of the isolated strain and its reaction mechanism on bovine hair could show biotechnological potential in the leather industry.Keywords
This publication has 32 references indexed in Scilit:
- Microbial keratinases and their prospective applications: an overviewApplied Microbiology and Biotechnology, 2006
- Specificities of proteases for use in leather manufactureJournal of Chemical Technology & Biotechnology, 2005
- Purification of keratinase from poultry farm isolate-Scopulariopsis brevicaulis and statistical optimization of enzyme activityEnzyme and Microbial Technology, 2005
- Production, purification and characterization of an extracellular keratinase from Lysobacter NCIMB 9497Letters in Applied Microbiology, 2002
- Purification and characterization of an alkaline serine endopeptidase from a feather-degradingXanthomonas maltophiliastrainCanadian Journal of Microbiology, 2002
- The analysis of merino wool cuticle and cortical cells by Fourier transform Raman spectroscopyPeptide Science, 1997
- Fourier-transform Raman spectroscopic study of human hairSpectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 1997
- Protease production by a thermophilic Bacillus species (P-001A) which degrades various kinds of fibrous proteinsBiotechnology Letters, 1993
- Amino acid sequence information in proteins and complex proteinaceous material revealed by pyrolysis-capillary gas chromatography-low and high resolution mass spectrometryJournal of Analytical and Applied Pyrolysis, 1987
- Sulfonyl Fluorides as Inhibitors of Esterases. II. Formation and Reactions of Phenylmethanesulfonyl α-Chymotrypsin*Biochemistry, 1964