Carboxy-Terminal Proteolytic Processing of Helicobacter pylori Vacuolating Toxin
- 1 January 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (1), 543-546
- https://doi.org/10.1128/iai.69.1.543-546.2001
Abstract
The vacA gene of Helicobacter pylori strain 60190 encodes a 1,287-amino-acid protoxin, which undergoes cleavage of a 33-amino-acid amino-terminal signal sequence and carboxy-terminal proteolytic processing to yield a mature secreted toxin. Several features of VacA suggest that it belongs to the autotransporter family of gram-negative bacterial secreted proteins. Based on matrix-assisted laser desorption ionization–time of flight mass spectrometric analysis, we calculate that the mature toxin has a mass of 88.2 ± 0.2 kDa and consists of approximately 821 amino acids.Keywords
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