The yeast orthologue of GRASP65 forms a complex with a coiled-coil protein that contributes to ER to Golgi traffic
Open Access
- 29 January 2007
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 176 (3), 255-261
- https://doi.org/10.1083/jcb.200607151
Abstract
The mammalian Golgi protein GRASP65 is required in assays that reconstitute cisternal stacking and vesicle tethering. Attached to membranes by an N-terminal myristoyl group, it recruits the coiled-coil protein GM130. The relevance of this system to budding yeasts has been unclear, as they lack an obvious orthologue of GM130, and their only GRASP65 relative (Grh1) lacks a myristoylation site and has even been suggested to act in a mitotic checkpoint. In this study, we show that Grh1 has an N-terminal amphipathic helix that is N-terminally acetylated and mediates association with the cis-Golgi. We find that Grh1 forms a complex with a previously uncharacterized coiled-coil protein, Ydl099w (Bug1). In addition, Grh1 interacts with the Sec23/24 component of the COPII coat. Neither Grh1 nor Bug1 are essential for growth, but biochemical assays and genetic interactions with known mediators of vesicle tethering (Uso1 and Ypt1) suggest that the Grh1–Bug1 complex contributes to a redundant network of interactions that mediates consumption of COPII vesicles and formation of the cis-Golgi.Keywords
This publication has 29 references indexed in Scilit:
- Global landscape of protein complexes in the yeast Saccharomyces cerevisiaeNature, 2006
- Proteome survey reveals modularity of the yeast cell machineryNature, 2006
- Exploration of the Function and Organization of the Yeast Early Secretory Pathway through an Epistatic Miniarray ProfileCell, 2005
- The Golgi-associated Protein GRASP65 Regulates Spindle Dynamics and Is Essential for Cell DivisionMolecular Biology of the Cell, 2005
- A Rab Requirement Is Not Bypassed inSLY1-20SuppressionMolecular Biology of the Cell, 2005
- The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-GolgiThe Journal of cell biology, 2004
- Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130The Journal of cell biology, 2001
- Evidence for Overlapping and Distinct Functions in Protein Transport of Coat Protein Sec24p Family MembersOnline Journal of Public Health Informatics, 2000
- Genetic Selection of Peptide Inhibitors of Biological PathwaysScience, 1999
- GRASP65, a Protein Involved in the Stacking of Golgi CisternaeCell, 1997