Tomographic molecular imaging and 3D quantification within adult mouse organs
- 3 December 2006
- journal article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 4 (1), 31-33
- https://doi.org/10.1038/nmeth985
Abstract
A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet beta-cell volume and the onset of type-1 diabetes.Keywords
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