Interaction Cloning: Identification of a Helix-Loop-Helix Zipper Protein that Interacts with c-Fos
- 15 May 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 256 (5059), 1014-1018
- https://doi.org/10.1126/science.1589769
Abstract
A facile method for isolating genes that encode interacting proteins has been developed with a polypeptide probe that contains an amino-terminal extension with recognition sites for a monoclonal antibody, a specific endopeptidase, and a site-specific protein kinase. This probe, containing the basic region-leucine zipper dimerization motif of c-Fos, was used to screen a complementary DNA library. A complementary DNA that encoded a member of the basic-helix-loop-helix-zipper (bHLH-Zip) family of proteins was isolated. The complementary DNA-encoded polypeptide FIP (Fos interacting protein) bound to oligonucleotide probes that contained DNA binding motifs for other HLH proteins. When cotransfected with c-Fos, FIP stimulated transcription of an AP-1-responsive promoter.Keywords
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