Influence of the Composition of the Cellulolytic Flora on the Development of Hydrogenotrophic Microorganisms, Hydrogen Utilization, and Methane Production in the Rumens of Gnotobiotically Reared Lambs
- 15 December 2010
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 76 (24), 7931-7937
- https://doi.org/10.1128/aem.01784-10
Abstract
We investigated the influence of the composition of the fibrolytic microbial community on the development and activities of hydrogen-utilizing microorganisms in the rumens of gnotobiotically reared lambs. Two groups of lambs were reared. The first group was inoculated with Fibrobacter succinogenes , a non-H 2 -producing species, as the main cellulolytic organism, and the second group was inoculated with Ruminococcus albus , Ruminococcus flavefaciens , and anaerobic fungi that produce hydrogen. The development of hydrogenotrophic bacterial communities, i.e., acetogens, fumarate and sulfate reducers, was monitored in the absence of methanogens and after inoculation of methanogens. Hydrogen production and utilization and methane production were measured in rumen content samples incubated in vitro in the presence of exogenous hydrogen (supplemented with fumarate or not supplemented with fumarate) or in the presence of ground alfalfa hay as a degradable substrate. Our results show that methane production was clearly reduced when the dominant fibrolytic species was a non-H 2 -producing species, such as Fibrobacter succinogenes , without significantly impairing fiber degradation and fermentations in the rumen. The addition of fumarate to the rumen contents stimulated H 2 utilization only by the ruminal microbiota inoculated with F. succinogenes , suggesting that these communities could play an important role in fumarate reduction in vivo .Keywords
This publication has 42 references indexed in Scilit:
- Microbial ecosystem and methanogenesis in ruminantsAnimal, 2010
- Establishment and Development of Ruminal Hydrogenotrophs in Methanogen-Free LambsApplied and Environmental Microbiology, 2007
- Degradation of Wheat Straw by Fibrobacter succinogenes S85: a Liquid- and Solid-State Nuclear Magnetic Resonance StudyApplied and Environmental Microbiology, 2005
- Interspecies H2 transfer in cellulose degradation between fibrolytic bacteria and H2-utilizing microorganisms from the human colonFEMS Microbiology Letters, 2001
- Methane production by ruminants: its contribution to global warmingAnimal Research, 2000
- Inhibition of the cellulolytic activity of Neocallimastix frontalis by Ruminococcus flavefaciensJournal of General Microbiology, 1993
- Establishment of ciliate protozoa in the rumen of conventional and conventionalized lambs: influence of diet and management conditionsCanadian Journal of Microbiology, 1988
- A simple and versatile micro-computer program for the determination of ‘most probable number’Journal of Microbiological Methods, 1983
- Studies on the Rumen Flagellate Neocallimastix frontalisJournal of General Microbiology, 1975
- The Cellulolytic Activity of Pure Strains of Bacteria from the Rumen of CattleJournal of General Microbiology, 1963