Transferability of SSR markers among wheat, rye, and triticale
- 5 December 2003
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 108 (6), 1147-1150
- https://doi.org/10.1007/s00122-003-1532-5
Abstract
Simple sequence repeat (SSR) markers are a valuable tool for many purposes, such as mapping, fingerprinting, and breeding. However, they are only available in some economically important crops because of the high cost and labor intensity involved in their development. Comparative mapping reveals a high degree of colinearity between closely related species, which allows the exchange of markers between them. Our objective was to examine the transferability of SSR markers among wheat (Triticum aestivum L.), rye (Secale cereale L.), and triticale (X Triticosecale Wittmack). One hundred forty-eight wheat and 28 rye SSR markers were used to amplify genomic DNA extracted from five lines each of wheat, rye, and triticale. Transferability of wheat SSR markers to rye was 17%, whereas 25% of rye markers were amplifiable in wheat. In triticale, 58% and 39% transferability was achieved for wheat and rye markers, respectively. Wheat markers gave an average of 2.6, 2.7, and 2.4 polymorphic bands in wheat, rye, and triticale, respectively, while rye markers gave an average of 2.0 in rye and none in wheat and triticale. These transferable markers can now be exploited for further genetic and breeding studies in these species.Keywords
This publication has 38 references indexed in Scilit:
- Development of microsatellite markers in peach [Prunus persica (L.) Batsch] and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.)Theoretical and Applied Genetics, 2002
- Identification of microsatellite polymorphisms in an expressed portion of the rye genomePlant Breeding, 2002
- Allopolyploidy-Induced Rapid Genome Evolution in the Wheat (Aegilops-Triticum) GroupPlant Cell, 2001
- Computational and Experimental Analysis of Microsatellites in Rice (Oryza sativa L.): Frequency, Length Variation, Transposon Associations, and Genetic Marker PotentialGenome Research, 2001
- Characterisation of microsatellite markers from sugarcane (Saccharum sp.), a highly polyploid speciesPlant Science, 2000
- Development of simple sequence repeat markers in rye (Secale cerealeL.)Genome, 1999
- An Integrated Genetic Linkage Map of the Soybean GenomeCrop Science, 1999
- Polyploidy: recurrent formation and genome evolutionTrends in Ecology & Evolution, 1999
- Utility of SSRs for Determining Genetic Similarities an Relationships in Maize Using an Agarose Gel SystemCrop Science, 1998
- High resolution of human evolutionary trees with polymorphic microsatellitesNature, 1994