Genetic Identification of Rickettsiae Isolated from Ticks in Japan
Open Access
- 1 June 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 40 (6), 2176-2181
- https://doi.org/10.1128/jcm.40.6.2176-2181.2002
Abstract
Following the description in Japan of Japanese spotted fever, caused by Rickettsia japonica , a search for the vector of this disease led to the isolation of several rickettsiae from various tick species. Sixty-three rickettsial isolates were obtained from six different tick species, and six type strains were described by PCR and monoclonal antibody testing. We identified these six strains by amplification and sequencing of the genes encoding 16S rRNA and citrate synthase. We confirmed that the isolates from Dermacentor taiwanensis and Haemaphysalis flava ticks were R. japonica isolates. In Ixodes ovatus , Ixodes persulcatus , and Ixodes monospinosus , we identified a Rickettsia identical or closely related to Rickettsia helvetica , a species that is pathogenic for humans and that to date has only been found in Europe. Finally, we identified a new genotype of unknown pathogenicity, genotype AT, that was isolated from Amblyomma testudinarium ticks and that is closely related to a Slovakian genotype obtained from Ixodes ricinus ticks.Keywords
This publication has 24 references indexed in Scilit:
- Ticks and Tickborne Bacterial Diseases in Humans: An Emerging Infectious ThreatClinical Infectious Diseases, 2001
- Evidence of Rickettsia helvetica infection in humans, eastern France.Emerging Infectious Diseases, 2000
- Ultrastructure of Spotted Fever Group Rickettsiae in Tissues of Larval Amblyomma testudinarium (Acari: Ixodidae).Journal of the Acarological Society of Japan, 2000
- Three human cases of tick bite caused by Ixodes persulcatus at high altitude in Shikoku island, Japan.Journal of the Acarological Society of Japan, 1998
- Japanese Spotted Fever: Report of 31 Cases and Review of the LiteratureEmerging Infectious Diseases, 1997
- Citrate Synthase Gene Comparison, a New Tool for Phylogenetic Analysis, and Its Application for the RickettsiaeInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Research in Microbiology, 1995
- Bootstrap hypothesis tests for evolutionary trees and other dendrograms.Proceedings of the National Academy of Sciences of the United States of America, 1994
- Rickettsia japonica sp. nov., the Etiological Agent of Spotted Fever Group Rickettsiosis in JapanInternational Journal of Systematic and Evolutionary Microbiology, 1992
- A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequencesJournal of Molecular Evolution, 1980