Relationships between arginine degradation, pH and survival inLactobacillus sakei
Open Access
- 1 November 1999
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 180 (2), 297-304
- https://doi.org/10.1111/j.1574-6968.1999.tb08809.x
Abstract
Lactobacillus sakei is one of the most important lactic acid bacteria of meat and fermented meat products. It is able to degrade arginine with ammonia and ATP production by the arginine deiminase pathway (ADI). This pathway is composed of three enzymes: arginine deiminase, ornithine transcarbamoylase and carbamate kinase, and an arginine transport system. The transcription of the ADI pathway is induced by arginine and subjected to catabolite repression. In order to understand the physiological role of the degradation of this amino acid we investigated the growth of bacteria under various conditions. We show that arginine degradation is responsible for an enhanced viability during the stationary phase when cells are grown under anaerobiosis. Arginine is necessary for the induction of the ADI pathway but in association with another environmental signal. Using a mutant of the l-lactate dehydrogenase unable to lower the pH we could clearly demonstrate that (i) low pH is not responsible for cell death during the stationary phase, so survival is due to another factor than elevated pH, (ii) neither low pH nor oxygen limitation is responsible for the induction of the ADI pathway together with arginine since the ldhL mutant is able to degrade arginine under aerobiosis.Keywords
This publication has 15 references indexed in Scilit:
- Disruption of the sole IdhL gene in Lactobacillus sakei prevents the production of both L- and D-IactateMicrobiology, 1998
- A REVIEW : Arginine metabolism in wine lactic acid bacteria and its practical significanceJournal of Applied Microbiology, 1998
- Taxonomic Note: Necessary Correction of Specific Epithets Formed as Substantives (Nouns) “in Apposition”International Journal of Systematic and Evolutionary Microbiology, 1997
- Collagenase gene (colA) is located in the 3′-flanking region of the perfringolysin O (pfoA) locus in Clostridium perfringensFEMS Microbiology Letters, 1997
- Fermentative arginine degradation in Halobacterium salinarium (formerly Halobacterium halobium): genes, gene products, and transcripts of the arcRACB gene clusterJournal of Bacteriology, 1996
- Catabolism of arginine byCarnobacterium spp. isolated from vacuum-packed sugar-salted fishCurrent Microbiology, 1994
- RNA processing modulates the expression of the arcDABC operon in Pseudomonas aeruginosaJournal of Molecular Biology, 1992
- A simplified key for identifying homofermentative Lactobacillus and Carnobacterium spp. from meatJournal of Applied Bacteriology, 1991
- Positive FNR-like control of anaerobic arginine degradation and nitrate respiration in Pseudomonas aeruginosaJournal of Bacteriology, 1991
- A MEDIUM FOR THE CULTIVATION OF LACTOBACILLIJournal of Applied Bacteriology, 1960