Measurement of Absorption and Scattering With an Integrating Sphere Detector: Application to Microalgae
- 1 March 2009
- journal article
- Published by National Institute of Standards and Technology (NIST) in Journal of Research of the National Institute of Standards and Technology
- Vol. 114 (2), 69-81
- https://doi.org/10.6028/jres.114.006
Abstract
A spectrometer with an integrating sphere (IS) detector was used to measure the absorbance due to scattering and absorption. Analysis of the measurement process showed that two measurements of the absorbance, one with the cuvette placed in the normal spectrometer position, and the second with the cuvette placed next to the entrance aperture of the IS detector, provide enough information to separate the contributions from scattering and molecular absorption. Measurements were carried out with mixtures of microsphere and chromophore solutions. Two cases were examined: microspheres suspended in an aqueous fluorescein solution, and microspheres suspended in an aqueous holmium oxide solution. In both cases, the proposed measurement model gave results which were in good agreement with the expected response. Measurements on microalgae suspensions yielded a molecular absorption contribution and a scattering contribution. The scattering contribution had significant spectral structure which was inversely related to the molecular absorption contribution. The absorption and scattering contributions may provide independent information on the status of chlorophyll molecules and the structure of chloroplasts in microalgae.Keywords
This publication has 12 references indexed in Scilit:
- Measurement of the fluorescence quantum yield using a spectrometer with an integrating sphere detectorJournal of Research of the National Institute of Standards and Technology, 2008
- Scattering properties of microalgae: the effect of cell size and cell wallApplied Optics, 2007
- Chlorophyll fluorescence—a practical guideJournal of Experimental Botany, 2000
- Depolarized Resonance Light Scattering by Porphyrin and Chlorophyll a AggregatesBiophysical Journal, 1998
- Absorption spectrum (380–700 nm) of pure water II Integrating cavity measurementsApplied Optics, 1997
- Use of freshwater plants for phytotoxicity testing: A reviewEnvironmental Pollution, 1995
- Calibration of an integrating sphere for determining the absorption coefficient of scattering suspensionsApplied Optics, 1993
- Selective scattering spectra as an approach to internal structure of granal and agranal chloroplasts.Proceedings of the National Academy of Sciences of the United States of America, 1977
- Absorption and Fluorescence of Chlorophyll a in Particle Fractions from Different PlantsBiophysical Journal, 1969
- Selective scattering of light by pigments in vivoArchives of Biochemistry and Biophysics, 1959