Characterization and identification of the major flavonoids in Phyllostachys edulis leaf extract by UPLC–QTOF–MS/MS
Open Access
- 1 December 2020
- journal article
- research article
- Published by Akademiai Kiado Zrt. in Acta Chromatographica
- Vol. 32 (4), 228-237
- https://doi.org/10.1556/1326.2019.00688
Abstract
Phyllostachys edulis (PES), the most important bamboo species in China, is widely distributed in East Asia. Flavonoids, which are important bioactive natural compounds, often have similar structures, making their structural elucidation difficult. The aim of this study was to represent valuable, reliable mass spectral data for the identification of flavonoids in plant leaves. Ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS/MS) method was established for characterization and identification of the major flavonoids in PES leaf extract. A total of 13 flavonoids were simultaneously characterized, and their proposed characteristic product ions and fragmentation pathways were investigated. Thirteen compounds were separated on an Agilent Zorbax RRHD SB-C18 column (150 mm × 2.1 mm, 1.8 μm). On the basis of comparing with the 4 reference standards and the literature data, the other 9 flavonoids were identified by tandem mass spectrometry (MS/MS). Eight compounds (compounds 1, 4, 5, 8, 9, 10, 11, and 12) were found in PES leaves for the first time. An efficient UPLC–QTOF-MS/MS method was successfully applied for the structural identification of flavonoids in PES leaves. These results have practical applications for the rapid identification and structural characterization of these compounds in crude bioactive extracts or mixtures. Phyllostachys edulis (PES), the most important bamboo species in China, is widely distributed in East Asia. Flavonoids, which are important bioactive natural compounds, often have similar structures, making their structural elucidation difficult. The aim of this study was to represent valuable, reliable mass spectral data for the identification of flavonoids in plant leaves. Ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC–Q-TOF-MS/MS) method was established for characterization and identification of the major flavonoids in PES leaf extract. A total of 13 flavonoids were simultaneously characterized, and their proposed characteristic product ions and fragmentation pathways were investigated. Thirteen compounds were separated on an Agilent Zorbax RRHD SB-C18 column (150 mm × 2.1 mm, 1.8 μm). On the basis of comparing with the 4 reference standards and the literature data, the other 9 flavonoids were identified by tandem mass spectrometry (MS/MS). Eight compounds (compounds 1, 4, 5, 8, 9, 10, 11, and 12) were found in PES leaves for the first time. An efficient UPLC–QTOF-MS/MS method was successfully applied for the structural identification of flavonoids in PES leaves. These results have practical applications for the rapid identification and structural characterization of these compounds in crude bioactive extracts or mixtures.Keywords
This publication has 46 references indexed in Scilit:
- Rapid resolution liquid chromatography–electrospray ionisation tandem mass spectrometry method for identification of chemical constituents in Citri Reticulatae PericarpiumFood Chemistry, 2013
- A comparison of C-glycosidic flavonoid isomers by electrospray ionization quadrupole time-of-flight tandem mass spectrometry in negative and positive ion modeInternational Journal of Mass Spectrometry, 2013
- HPLC–ESI-Q-TOF-MS for a comprehensive characterization of bioactive phenolic compounds in cucumber whole fruit extractFood Research International, 2012
- Profiling Analysis of the Seeds of Vaccaria segetalis (Necr.) Gracke by HPLC-ESI-MSAdvanced Materials Research, 2011
- Identification of phenolic compounds in aqueous and ethanolic rooibos extracts (Aspalathus linearis) by HPLC-ESI-MS (TOF/IT)Analytical and Bioanalytical Chemistry, 2011
- Structural characterization of flavonoid glycosides by multi‐stage mass spectrometryMass Spectrometry Reviews, 2008
- Antioxidant C-glycosyl flavones from the leaves of Sasa kurilensis var. giganteaPhytochemistry, 2008
- Mass spectrometry in the structural analysis of flavonoidsJournal of Mass Spectrometry, 2004
- Mass spectrometric methods for the analysis of chlorinated and nitrated isoflavonoids: a novel class of biological metabolitesJournal of Mass Spectrometry, 2003
- A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugatesGlycoconjugate Journal, 1988