Emergence of novel European genotype porcine reproductive and respiratory syndrome virus in mainland China
- 7 January 2011
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 92 (4), 880-892
- https://doi.org/10.1099/vir.0.027995-0
Abstract
Porcine reproductive and respiratory syndrome (PRRS) has a major negative economic impact on the swine industry worldwide. During the investigation of PRRS virus (PRRSV) in mainland China, European genotype (EU, type 1) PRRSV isolates were detected in swine herds both with and without clinical symptoms. Two complete genome sequences for Chinese type 1 PRRSV isolates were identified from viruses isolated from lung tissue and sera. The two viruses, designated BJEU06-1 and NMEU09-1, produced cytopathic effects in primary porcine alveolar macrophages but not in Marc-145 cells, and had a mean diameter of 55 nm, as measured by transmission electron microscopy . Comparative sequence analysis revealed that they shared 87.0–91.5 % and 58.0–58.2 % identity with the EU and North American genotype (NA, type 2) prototypic strains LV and VR-2332, respectively. Remarkably, these isolates, characterized by concomitant deletions within non-structural protein 2 (Nsp2) and ORF3 hypervariable regions, have never been described. Phylogenetic trees showed that all of the novel Chinese isolates of European genotype are in the pan-European subtype 1 that is predominant in Europe. However, they evolved from different ancestors. These novel viruses are predicted to be products of the divergent evolution of ancestor PRRSV isolates introduced from Europe. This is the first report of type 1 PRRSV wild isolates being in mainland China. Our findings confirm that the Chinese type 1 PRRSV isolates originated from diverse progenitors and the type 1 and type 2 PRRSV isolates, having different biological properties, have coexisted on the Chinese mainland for several years.Keywords
This publication has 70 references indexed in Scilit:
- The PRRSV replicase: Exploring the multifunctionality of an intriguing set of nonstructural proteinsVirus Research, 2010
- Rapid differential detection of classical and highly pathogenic North American Porcine Reproductive and Respiratory Syndrome virus in China by a duplex real-time RT-PCRJournal of Virological Methods, 2009
- Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune responseJournal of General Virology, 2009
- Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)Virology Journal, 2009
- Genetic diversity of porcine reproductive and respiratory syndrome virus strains circulating in Hungarian swine herdsVeterinary Microbiology, 2008
- Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virusVirology, 2006
- Heterogeneity in Nsp2 of European-like porcine reproductive and respiratory syndrome viruses isolated in the United StatesVirus Research, 2004
- North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions.Journal of General Virology, 1999
- Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus, a Member of the Arterivirus GroupVirology, 1993
- Isolation of Swine Infertility and Respiratory Syndrome Virus (Isolate ATCC VR-2332) in North America and Experimental Reproduction of the Disease in Gnotobiotic PigsJournal of Veterinary Diagnostic Investigation, 1992