Regulation of imprinted gene expression in Arabidopsis endosperm
Open Access
- 21 January 2011
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 108 (5), 1755-1762
- https://doi.org/10.1073/pnas.1019273108
Abstract
Imprinted genes are expressed primarily or exclusively from either the maternal or paternal allele, a phenomenon that occurs in flowering plants and mammals. Flowering plant imprinted gene expression has been described primarily in endosperm, a terminal nutritive tissue consumed by the embryo during seed development or after germination. Imprinted expression in Arabidopsis thaliana endosperm is orchestrated by differences in cytosine DNA methylation between the paternal and maternal genomes as well as by Polycomb group proteins. Currently, only 11 imprinted A. thaliana genes are known. Here, we use extensive sequencing of cDNA libraries to identify 9 paternally expressed and 34 maternally expressed imprinted genes in A. thaliana endosperm that are regulated by the DNA-demethylating glycosylase DEMETER, the DNA methyltransferase MET1, and/or the core Polycomb group protein FIE. These genes encode transcription factors, proteins involved in hormone signaling, components of the ubiquitin protein degradation pathway, regulators of histone and DNA methylation, and small RNA pathway proteins. We also identify maternally expressed genes that may be regulated by unknown mechanisms or deposited from maternal tissues. We did not detect any imprinted genes in the embryo. Our results show that imprinted gene expression is an extensive mechanistically complex phenomenon that likely affects multiple aspects of seed development.Keywords
This publication has 53 references indexed in Scilit:
- Global Epigenetic and Transcriptional Trends among Two Rice Subspecies and Their Reciprocal HybridsTHE PLANT CELL ONLINE, 2010
- Ethylene signaling and response: where different regulatory modules meetCurrent Opinion in Plant Biology, 2009
- Diversity of Polycomb group complexes in plants: same rules, different players?Trends in Genetics, 2009
- The SRA domain of UHRF1 flips 5-methylcytosine out of the DNA helixNature, 2008
- MATERNALLY EXPRESSED PAB C-TERMINAL, a Novel Imprinted Gene inArabidopsis, Encodes the Conserved C-Terminal Domain of Polyadenylate Binding ProteinsTHE PLANT CELL ONLINE, 2008
- Tandem repeats upstream of the Arabidopsis endogene SDC recruit non-CG DNA methylation and initiate siRNA spreadingGenes & Development, 2008
- The SRA Methyl-Cytosine-Binding Domain Links DNA and Histone MethylationCurrent Biology, 2007
- Functional Diversification of Acyl-Coenzyme A Oxidases in Jasmonic Acid Biosynthesis and ActionPlant Physiology, 2006
- Partially redundant functions of two SET-domain polycomb-group proteins in controlling initiation of seed development in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2006
- Maintenance of DNA Methylation during theArabidopsisLife Cycle Is Essential for Parental ImprintingTHE PLANT CELL ONLINE, 2006