Role of Sulfated Glycans in Adherence of the Microsporidian Encephalitozoon intestinalis to Host Cells In Vitro
Open Access
- 1 February 2005
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 73 (2), 841-848
- https://doi.org/10.1128/iai.73.2.841-848.2005
Abstract
Microsporidia are obligate intracellular opportunistic protists that infect a wide variety of animals, including humans, via environmentally resistant spores. Infection requires that spores be in close proximity to host cells so that the hollow polar tube can pierce the cell membrane and inject the spore contents into the cell cytoplasm. Like other eukaryotic microbes, microsporidia may use specific mechanisms for adherence in order to achieve target cell proximity and increase the likelihood of successful infection. Our data show that Encephalitozoon intestinalis exploits sulfated glycans such as the cell surface glycosaminoglycans (GAGs) in selection of and attachment to host cells. When exogenous sulfated glycans are used as inhibitors in spore adherence assays, E. intestinalis spore adherence is reduced by as much as 88%. However, there is no inhibition when nonsulfated glycans are used, suggesting that E. intestinalis spores utilize sulfated host cell glycans in adherence. These studies were confirmed by exposure of host cells to xylopyranoside, which limits host cell surface GAGs, and sodium chlorate, which decreases surface sulfation. Spore adherence studies with CHO mutant cell lines that are deficient in either surface GAGs or surface heparan sulfate also confirmed the necessity of sulfated glycans. Furthermore, when spore adherence is inhibited, host cell infection is reduced, indicating a direct association between spore adherence and infectivity. These data show that E. intestinalis specifically adheres to target cells by way of sulfated host cell surface GAGs and that this mechanism serves to enhance infectivity.Keywords
This publication has 44 references indexed in Scilit:
- Microsporidiosis in Travelers with Diarrhea from the TropicsJournal of Travel Medicine, 1999
- Functions of Cell Surface Heparan Sulfate ProteoglycansAnnual Review of Biochemistry, 1999
- German Section Society of Protozoologists 17th Annual MeetingThe Journal of Eukaryotic Microbiology, 1999
- Scandinavian Section Society of Protozoologists 20th Annual MeetingThe Journal of Eukaryotic Microbiology, 1999
- Intestinal Microsporidiosis in a HIV-Seronegative PatientScandinavian Journal of Infectious Diseases, 1998
- Inhibition of the Spore Polar Filament Extrusion of the Microsporidium, Encephalitozoon hellem, Isolated from an AIDS Patient1The Journal of Eukaryotic Microbiology, 1993
- Effects of β-d-xyloside on morphogenesis and cytodifferentiation in cultured embryonic mouse molarsCell Differentiation and Development, 1990
- Tumor Formation Dependent on Proteoglycan BiosynthesisScience, 1988
- Chlorate: A reversible inhibitor of proteoglycan sulfationBiochemical and Biophysical Research Communications, 1988
- Phagocytized Intracellular Microsporidian Blocks Phagosome Acidification and Phagosome‐Lysosome FusionThe Journal of Protozoology, 1985